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In vitro methylation of the BsuRI (5'-GGCC-3') sites in the E2a region of adenovirus type 2 DNA does not affect expression in Xenopus laevis oocytes.
Vardimon L
,
Günthert U
,
Doerfler W
.
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The early region 2a (E2a) of adenovirus type 2 (Ad2) DNA codes for a 72,000-dalton DNA-binding protein and is expressed in the Ad2-transformed hamster cell line HE1 but not in cell lines HE2 and HE3 (H. Esche, J. Virol. 41:1076-1082, 1982; K. Johansson et al., J. Virol. 27:628-639, 1978). An inverse correlation between DNA methylation at the 5'-CCGG-3' sites of the E2a region and of gene expression in these cell lines has been observed (L. Vardimon et al., Nucleic Acids Res. 8:2461-2473, 1980). When the cloned E2a region of Ad2 DNA is methylated in vitro at the 5'-CCGG-3' sites, the gene is not transcribed after being injected into the nuclei of Xenopus laevis oocytes, whereas unmethylated DNA is expressed (L. Vardimon et al., Eur. J. Cell Biol. 25:13-15, 1981; L. Vardimon et al., Proc. Natl. Acad. Sci. U.S.A. 79:1073-1077, 1982). These data demonstrate that DNA methylation is directly involved in the shut-off of transcription. In the present communication we investigated in detail the control region of the gene for the DNA-binding protein in Ad2-transformed cell lines and showed that the first late control region (map coordinate 72 on the viral DNA) of the E2a region is present in its entirety in cell lines HE1, HE2, and HE3. The HaeIII sites (5'-GGCC-3') in the E2a region in all three cell lines were not methylated. When the DNA methyltransferase BsuRI was used, all 5'-GGCC-3' sites in the cloned E2a region of Ad2 DNA were methylated in vitro. It was shown that methylation of these sites did not inhibit the expression of this viral gene in X. laevis oocytes. Thus, for methylation to affect gene expression in the E2a region it has to occur at specific sites (e.g., 5'-CCGG-3') which may be different for other genes.
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977, Pubmed
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977,
Pubmed
Baker,
Promoters and heterogeneous 5' termini of the messenger RNAs of adenovirus serotype 2.
1981,
Pubmed
Chow,
Complex splicing patterns of RNAs from the early regions of adenovirus-2.
1979,
Pubmed
Cook,
Host response to adenovirus 2-transformed hamster embryo cells.
1979,
Pubmed
Ehrlich,
5-Methylcytosine in eukaryotic DNA.
1981,
Pubmed
Esche,
Viral gene products in adenovirus type-2 transformed hamster cells.
1982,
Pubmed
Galibert,
Nucleotide sequence of the EcoRI-F fragment of adenovirus 2 genome.
1979,
Pubmed
Goldenberg,
Coordinate regulation of two cytoplasmic RNA species transcribed from early region 2 of the adenovirus 2 genome.
1981,
Pubmed
Grippo,
Methylation of DNA in developing sea urchin embryos.
1968,
Pubmed
Gunthert,
DNA methylation in adenovirus, adenovirus-transformed cells, and host cells.
1976,
Pubmed
Günthert,
Restriction and modification in Bacillus subtilis: two DNA methyltransferases with BsuRI specificity. I. Purification and physical properties.
1981,
Pubmed
Günthert,
Restriction and modification in Bacillus subtilis: two DNA methyltransferases with BsuRI specificity. II. Catalytic properties, substrate specificity, and mode of action.
1981,
Pubmed
Johansson,
Viral DNA sequences and gene products in hamster cells transformed by adenovirus type 2.
1978,
Pubmed
Kruczek,
The unmethylated state of the promoter/leader and 5'-regions of integrated adenovirus genes correlates with gene expression.
1982,
Pubmed
,
Xenbase
Mann,
Specificity of Hpa II and Hae III DNA methylases.
1977,
Pubmed
Razin,
DNA methylation and gene function.
1980,
Pubmed
Razin,
DNA methylation and its possible biological roles.
1981,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Schirm,
Expression of viral DNA in adenovirus type 12-transformed cells, in tumor cells, and in revertants.
1981,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Stein,
In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.
1982,
Pubmed
Sutter,
Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression.
1980,
Pubmed
Sutter,
Methylation of integrated viral DNA sequences in hamster cells transformed by adenovirus 12.
1980,
Pubmed
Vardimon,
Expression of a cloned adenovirus gene is inhibited by in vitro methylation.
1982,
Pubmed
,
Xenbase
Vardimon,
DNA methylation and viral gene expression in adenovirus-transformed and -infected cells.
1980,
Pubmed
Vardimon,
Methylation of adenovirus genes in transformed cells and in vitro: influence on the regulation of gene expression?
1981,
Pubmed
,
Xenbase
Vardimon,
Patterns of integration of viral DNA in adenovirus type 2-transformed hamster cells.
1981,
Pubmed
Waechter,
Effect of methylation on expression of microinjected genes.
1982,
Pubmed
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Wigler,
The inheritance of methylation patterns in vertebrates.
1981,
Pubmed
