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Sequences of four tRNA genes from Caenorhabditis elegans and the expression of C. elegans tRNALeu (anticodon IAG) in Xenopus oocytes.
Tranquilla TA
,
Cortese R
,
Melton D
,
Smith JD
.
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Four tRNA genes have been identified in cloned segments of Caenorhabditis elegans DNA by tRNA hybridisation and expression after injection into Xenopus laevis oocyte nuclei. From DNA sequencing these are (with DNA anticodon sequences) tRNAAsp (GTC), tRNALeu (AAG), tRNALys (CTT) and tRNAPro (TGG). Their flanking DNA sequences are compared. Two identical tRNALys (CTT) genes from different regions of the genome have quite unrelated 5' flanking sequences. The tRNA synthesised in Xenopus oocytes after injection of the tRNALeu cloned DNA has the modified anticodon IAG. The tRNALeu gene precursor transcript from injected oocytes has short 5' and 3' additional sequences and lacks certain of those modified bases found in the processed tRNA.
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977, Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Ciliberto,
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1982,
Pubmed
,
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Clewell,
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1972,
Pubmed
Cortese,
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1978,
Pubmed
,
Xenbase
De Robertis,
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1979,
Pubmed
,
Xenbase
DeFranco,
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1980,
Pubmed
,
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DeFranco,
Identification of regulatory sequences contained in the 5'-flanking region of Drosophila lysine tRNA2 genes.
1981,
Pubmed
Duckworth,
Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
1981,
Pubmed
Duester,
The distal end of the ribosomal RNA operon rrnD of Escherichia coli contains a tRNA1thr gene, two 5s rRNA genes and a transcription terminator.
1980,
Pubmed
England,
Enzymatic oligoribonucleotide synthesis with T4 RNA ligase.
1978,
Pubmed
Feldmann,
Analyses of some oligonucleotide sequences and odd nucleotides from serine transfer ribonucleic acids.
1966,
Pubmed
Garber,
Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro.
1979,
Pubmed
Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase
Hovemann,
Analysis of a drosophila tRNA gene cluster.
1980,
Pubmed
Karn,
Novel bacteriophage lambda cloning vector.
1980,
Pubmed
Kole,
Properties of purified ribonuclease P from Escherichia coli.
1981,
Pubmed
Koski,
Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
1980,
Pubmed
,
Xenbase
Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Melton,
Transcription of cloned tRNA genes and the nuclear partitioning of a tRNA precursor.
1979,
Pubmed
,
Xenbase
Nishimura,
Minor components in transfer RNA: their characterization, location, and function.
1972,
Pubmed
Robertson,
Purification and properties of a specific Escherichia coli ribonuclease which cleaves a tyrosine transfer ribonucleic acid presursor.
1972,
Pubmed
Robinson,
Analysis of a drosophila tRNA gene cluster: two tRNALeu genes contain intervening sequences.
1981,
Pubmed
Rothstein,
Synthetic adaptors for cloning DNA.
1979,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Silverman,
The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.
1979,
Pubmed
,
Xenbase
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Sprague,
5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.
1980,
Pubmed
,
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Yen,
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1980,
Pubmed
,
Xenbase
