Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-30233
Biosci Rep 1983 May 01;35:461-7.
Show Gene links Show Anatomy links

Direct radioactive labelling of poly(ADP-ribose) in developing Xenopus laevis embryos.

Williams GT , Shall S , Ford CC .


???displayArticle.abstract???
It has not previously been possible to label the nuclear protein modification poly(ADP-ribose) directly from NAD because of the impermeability of the cell membrane. We have overcome this important problem by micro-injection of radioactively labelled NAD into Xenopus laevis early embryos. The polymer was identified and then quantified by its insensitivity to DNAase, RNAase, and spleen phosphodiesterase and by the chromatographic mobility of the products of digestion with snake-venom phosphodiesterase. The quantity of poly(ADP-ribose) present after 25 h of development (129 ng/mg DNA) is lower than that found in fully differentiated tissue.

???displayArticle.pubmedLink??? 6309267
???displayArticle.link??? Biosci Rep