Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-30048
Biochim Biophys Acta 1983 Oct 13;7411:109-15.
Show Gene links Show Anatomy links

Preliminary characterisation of inhibitors of DNA polymerase isolated from Xenopus laevis early embryos.

Smith C , McKune K , Cox R , Ford C .


???displayArticle.abstract???
Inhibitors of DNA polymerase have been detected in Xenopus laevis ovary and egg extracts. The characteristics of the inhibitors differ between the two extracts. In ovary preparations, the inhibitor is retained by dialysis tubing and is heat sensitive, whereas in egg extracts it is diffusable and heat stable. In both extracts, the activity co-elutes with DNA polymerase after ion exchange chromatography. Chromatography of ovary extracts renders the inhibitor diffusable and heat stable. Preliminary characterisation of inhibitory activity from eggs shows that the substance is sensitive to pronase digestion and has an approx. 300-500 molecular weight. Kinetic studies demonstrate that the inhibitor is uncompetitive with the DNA template and show mixed inhibitory kinetics with respect to the deoxynucleotides.

???displayArticle.pubmedLink??? 6615840
???displayArticle.link??? Biochim Biophys Acta