Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Isolation and translation of mRNA coding for the variant surface antigens of Paramecium.
Sommerville J
.
???displayArticle.abstract???
In the poly(A)+RNA isolated from the ciliate Paramecium primaurelia is found a discrete and abundant mRNA species of high molecular weight (corresponding to about 9,000 nucleotides). This mRNA species has size and abundance characteristics that identify it tentatively as the message coding for the variant cell-surface antigens. After microinjection of the high molecular weight mRNA into amphibian oocytes, polypeptides are synthesized that are immunoprecipitated specifically with antibodies directed against the homologous Paramecium antigen. On collecting the culture medium of oocytes microinjected with Paramecium mRNA, newly-synthesized complete antigen molecules (Mr approximately 300,000) can be recovered by immunoprecipitation.
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976, Pubmed,
Xenbase
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976,
Pubmed
,
Xenbase
Bolivar,
Immunological crossreactivity of Paramecium surface antigens: conformational differences account for cell surface specificity.
1983,
Pubmed
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Glisin,
Ribonucleic acid isolated by cesium chloride centrifugation.
1974,
Pubmed
Hansma,
The immobilization antigen of Paramecium aurelia is a single polypeptide chain.
1975,
Pubmed
JONES,
STUDIES ON THE CHARACTERIZATION AND STRUCTURE OF THE IMMOBILIZATION ANTIGENS OF PARAMECIUM AURELIA.
1965,
Pubmed
Kloetzel,
Analysis of reconstruction of an RNP particle which stores 5S RNA and tRNA in amphibian oocytes.
1981,
Pubmed
Lane,
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
1980,
Pubmed
,
Xenbase
Macindoe,
Adsorption titration as a specific semi-quantitative assay for soluble and bound Paramecium serotypic antigen.
1967,
Pubmed
McMaster,
Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
1977,
Pubmed
McTavish,
Macronuclear DNA organization and transcription in Paramecium primaurelia.
1980,
Pubmed
Preer,
mRNAs for the immobilization antigens of Paramecium.
1981,
Pubmed
Reisner,
The largest known monomeric globular proteins.
1969,
Pubmed
Rungger,
DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
STEERS,
A comparison of the tryptic peptides obtained from immobilization antigens of Paramecium aurelia.
1962,
Pubmed
Sommerville,
The effect of temperature change on gene expression in Paramecium primaurelia.
1982,
Pubmed
Sommerville,
Serotype transformation in Paramecium aurelia: antigen synthesis after a temperature change.
1969,
Pubmed
Sommerville,
Immobilization antigen synthesis in Paramecium aurelia synthesis in a cell-free amino acid incorporating system.
1970,
Pubmed
