Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-29962
J Chromatogr 1983 Dec 23;281:159-66. doi: 10.1016/s0021-9673(01)87875-4.
Show Gene links Show Anatomy links

Gel permeation, ion-exchange and reversed-phase columns for separation of metallothioneins by high-performance liquid chromatography-atomic absorption spectrophotometry.

Suzuki KT , Sunaga H , Aoki Y , Yamamura M .


???displayArticle.abstract???
Metallothioneins were separated on three kinds of HPLC column that differ in separation principle: gel permeation, ion exchange and reversed phase. Elution of the columns at neutral to weakly basic buffer conditions prevented dissociation of the metals, and the metals bound to metallothioneins were quantitatively eluted out. The metals in the eluate were continuously determined on an atomic absorption spectrophotometer by introducing the eluate directly to the nebulizer tube. Prerequisites for the on-line separation-detection system are discussed.

???displayArticle.pubmedLink??? 6668332
???displayArticle.link??? J Chromatogr