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XB-ART-29939
Biol Cell 1984 Jan 01;503:223-8. doi: 10.1111/j.1768-322x.1984.tb00269.x.
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[Expression of hepatitis B virus (HBV) genome in the oocytes of two species of amphibians].

Larive-Zerbani A , Gallien CL , Pourcel C .


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Xenopus laevis and Pleurodeles waltlii oocyte nuclei are injected with a recombinant plasmid pCP 10, carrying two hepatitis B virus (HBV) genomes in a tandem arrangement. Transcription occurs in oocytes of both species and production of viral RNAs is demonstrated; better quantitative results are obtained with P. waltlii oocytes. Two viral proteins are detected: Hepatitis B surface antigen (Ag HBs) and Hepatitis B core antigen (Ag HBc). These proteins can be shown only in oocyte homogenates, and not in the incubation media. Another viral marker (Ag HBe) was not detected.

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