Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Interaction of metabolic inhibitors with actin fibrils.
Bereiter-Hahn J
,
Tillmann U
,
Vöth M
.
???displayArticle.abstract???
The dependence of the arrangement of fibrillar actin in cultured endothelial cells on metabolic conditions was investigated with cellular elements derived from the heart of Xenopus laevis tadpoles. Either primary culture or an established cell line (XTH-2) were used in these studies. The metabolic stage of the cells was influenced by inhibiting respiration and lactate production. The actin pattern was revealed either by indirect immunofluorescence or by tetramethylrhodaminyl (TRITC)-phalloidin fluorescence. Total block of energy supply causes in all cases a distinct loss of actin fibrils, while inhibition of respiration alone increases the variability of actin organization. In primary XTH cells but not in XTH-2 cells cyanide disintegrates most of the actin fibres during 3 h of treatment. This effect is independent of the inhibition of respiration, since actin gels prepared from skeletal muscle also undergo destruction in the presence of cyanide. It is concluded that the actin fibrils of the primary cells and the established line behave differently to changing metabolic conditions and to application of KCN.
Bereiter-Hahn,
Locomotion of Xenopus epidermis cells in primary culture.
1981, Pubmed,
Xenbase
Bereiter-Hahn,
Locomotion of Xenopus epidermis cells in primary culture.
1981,
Pubmed
,
Xenbase
Bershadsky,
ATP-dependent regulation of cytoplasmic microtubule disassembly.
1981,
Pubmed
Bershadsky,
Role of ATP in the regulation of stability of cytoskeletal structures.
1983,
Pubmed
Bershadsky,
Destruction of microfilament bundles in mouse embryo fibroblasts treated with inhibitors of energy metabolism.
1980,
Pubmed
Brinkley,
Rotenone inhibition of spindle microtubule assembly in mammalian cells.
1974,
Pubmed
Carley,
F-actin aggregates in transformed cells.
1981,
Pubmed
Faulstich,
Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis.
1983,
Pubmed
Korohoda,
Energy metabolic regulation of oscillatory contraction activity in Physarum polycephalum.
1983,
Pubmed
Meisner,
Metaphase arrest of Chinese hamster cells with rotenone.
1966,
Pubmed
ROSE,
A cellophane-strip technique for culturing tissue in multipurpose culture chambers.
1958,
Pubmed
Schlage,
A microscope perfusion respirometer for continuous respiration measurement of cultured cells during microscopic observation.
1983,
Pubmed
,
Xenbase
Vasiliev,
Spreading and locomotion of tissue cells: factors controlling the distribution of pseudopodia.
1982,
Pubmed
Weber,
Distribution of actin and tubulin in cells and in glycerinated cell models after treatment with cytochalasin B (CB).
1976,
Pubmed
Weber,
Griseofulvin interacts with microtubules both in vivo and in vitro.
1976,
Pubmed
Webster,
Visualization of the same PtK2 cytoskeletons by both immunofluorescence and low power electron microscopy.
1978,
Pubmed
Wehland,
Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth.
1977,
Pubmed
von Olenhusen,
Evidence for actin transformation during the contraction-relaxation cycle of cytoplasmic actomyosin: cycle blockade by phalloidin injection.
1979,
Pubmed