Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Structure and expression in Escherichia coli of a cloned rat interferon-alpha gene.
Dijkema R
,
Pouwels P
,
de Reus A
,
Schellekens H
.
???displayArticle.abstract???
DNA synthesized by in vitro transcription on rat interferon (IFN) mRNA has been cloned and amplified as recombinant DNA. The nucleotide sequence of these rat IFN cDNA clones revealed i. the partial presence of the coding region of the gene and ii all cDNA clones were derived from the same subtype of rat IFN-alpha mRNA. Purified inserted fragments were used as a hybridisation probe against chromosomal "Southern blots" to show that at least twelve rat IFN-alpha-related sequences are present in the genome. A lambda-linked rat gene library was screened with the cDNA probes, resulting in an equivalent number of rat IFN-alpha-related hybrid phages. By use of a 3'-noncoding region as a probe, the chromosomal counterpart of the cDNA clones could be detected and the nucleotide sequence of its coding region has been determined. Expression of the coding region in E. coli yielded biologically active IFN, when tested for in vitro or in vivo antiviral activity.
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977, Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bolivar,
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
1977,
Pubmed
Cantell,
Human leukocyte interferon: production, purification, stability, and animal experiments.
1974,
Pubmed
Derynck,
Expression of human fibroblast interferon gene in Escherichia coli.
1980,
Pubmed
Derynck,
Isolation and structure of a human fibroblast interferon gene.
1980,
Pubmed
Gannon,
Organisation and sequences at the 5' end of a cloned complete ovalbumin gene.
1979,
Pubmed
Goeddel,
The structure of eight distinct cloned human leukocyte interferon cDNAs.
1981,
Pubmed
Goeddel,
Human leukocyte interferon produced by E. coli is biologically active.
1980,
Pubmed
Gray,
Structure of the human immune interferon gene.
1982,
Pubmed
Gray,
Expression of human immune interferon cDNA in E. coli and monkey cells.
1982,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Hanahan,
Plasmid screening at high colony density.
1980,
Pubmed
Houghton,
The absence of introns within a human fibroblast interferon gene.
1981,
Pubmed
ISAACS,
Virus interference. I. The interferon.
1957,
Pubmed
Lawn,
Human fibroblast interferon gene lacks introns.
1981,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Nagata,
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity.
1980,
Pubmed
Nagata,
The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha.
1980,
Pubmed
Ohno,
Structure of a chromosomal gene for human interferon beta.
1981,
Pubmed
Sagar,
Multiple human beta interferon genes.
1982,
Pubmed
Sargent,
The rat serum albumin gene: analysis of cloned sequences.
1979,
Pubmed
Schellekens,
Production and initial characterization of rat interferon.
1980,
Pubmed
Schellekens,
Comparative antiviral efficiency of leukocyte and bacterially produced human alpha-interferon in rhesus monkeys.
1981,
Pubmed
Schellekens,
Antiviral and side effects of interferons produced by recombinant DNA techniques as tested in rhesus monkeys.
1982,
Pubmed
Shaw,
Structure and expression of cloned murine IFN-alpha genes.
1983,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Taira,
Mouse interferons: amino terminal amino acid sequences of various species.
1980,
Pubmed
Wieringa,
Purification of vitellogenin mRNA and serum albumin mRNA from avian liver by preparative gel electrophoresis.
1978,
Pubmed
