Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We have constructed a set of plasmids carrying a tRNAPro gene from C. elegans in a head to tail dimeric and trimeric arrangement with virtually no spacer sequence. We show that in two different transcriptional systems, each coding region functions as an internal promoter directing the synthesis of independent transcriptional products. This is in contrast with the property of natural head to tail dimeric arrangements found in yeast, where only one coding region functions as promoter (Mao et al., 1980 Cell 20, 589; Schmidt et al., 1980 Nature 287, 750). The evolutionary significance of our finding is discussed.
Barnes,
Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition.
1983, Pubmed
Barnes,
Kilo-sequencing: an ordered strategy for rapid DNA sequence data acquisition.
1983,
Pubmed
Castagnoli,
Processing of eukaryotic tRNA precursors: secondary structure of the precursor specific sequences affects the rate but not the accuracy of processing reactions.
1982,
Pubmed
Ciliberto,
Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
1982,
Pubmed
,
Xenbase
Ciliberto,
Relationship between the two components of the split promoter of eukaryotic tRNA genes.
1982,
Pubmed
,
Xenbase
Ciliberto,
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
1983,
Pubmed
,
Xenbase
Ciliberto,
Transcription by RNA polymerase III.
1983,
Pubmed
Cortese,
Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
1980,
Pubmed
,
Xenbase
Cortese,
Cloning of nematode tRNA genes and their expression in the frog oocyte.
1978,
Pubmed
,
Xenbase
Hall,
Transcription initiation of eucaryotic transfer RNA genes.
1982,
Pubmed
Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase
Mao,
Dimeric transfer RNA precursors in S. pombe.
1980,
Pubmed
,
Xenbase
Melton,
Transcription of cloned tRNA genes and the nuclear partitioning of a tRNA precursor.
1979,
Pubmed
,
Xenbase
Messing,
Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
1977,
Pubmed
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmidt,
Dimeric tRNA precursors in yeast.
1980,
Pubmed
,
Xenbase
Traboni,
A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
1982,
Pubmed
,
Xenbase
Traboni,
A general method to select for M13 clones carrying base pair substitution mutants constructed in vitro.
1983,
Pubmed
Weil,
Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
1979,
Pubmed