Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Phosphorylation of C-protein in intact amphibian cardiac muscle. Correlation between 32P incorporation and twitch relaxation.
Hartzell HC
.
???displayArticle.abstract???
The molecular mechanisms by which neurotransmitters modulate the force of contraction of cardiac muscle are incompletely understood. Hartzell and Titus (1982. J. Biol. Chem. 257:2111-2120) have recently reported that C-protein, an integral component of the thick filament, is reversibly phosphorylated in response to ionotropic agents. In this communication, C-protein phosphorylation (as measured by isotopic labeling with 32P) is correlated with changes in the rate of relaxation of twitch tension. On the average, isoproterenol simultaneously increases peak systolic tension twofold, decreases twitch relaxation time from a control value of approximately 450 to approximately 300 ms, and increases C-protein phosphorylation two- to threefold, with a maximum effect occurring less than 60 s after addition of 1 microM isoproterenol. Carbamylcholine, in contrast, decreases peak systolic tension more rapidly than it affects relaxation or C-protein phosphorylation. The maximum decrease in peak tension (60%) occurs within 1 min of addition of 0.5 microM carbamylcholine, but relaxation time increases slowly to 800 ms over approximately 6 min. The increase in relaxation time correlates well with the decrease in 32P incorporation into C-protein (r = 0.94). Changing beat frequency between 0.2 and 1/s has no effect on C-protein phosphorylation but does alter relaxation time (relaxation time decreases approximately 100 ms when beat frequency is changed from 0.5 to 1/s) and thus alters the quantitative relationship between C-protein phosphorylation and relaxation rate. These results suggest that two separate processes affect relaxation. It is proposed that the level of C-protein phosphorylation sets the boundaries over which relaxation is regulated by a second process that is dependent upon beat frequency and probably involves changes in intracellular Ca.
Allen,
The effects of muscle length on intracellular calcium transients in mammalian cardiac muscle.
1982, Pubmed
Allen,
The effects of muscle length on intracellular calcium transients in mammalian cardiac muscle.
1982,
Pubmed
Allen,
Calcium transients in aequorin-injected frog cardiac muscle.
1978,
Pubmed
Anderson,
Mechanism of activation of contraction in frog ventricular muscle.
1977,
Pubmed
Bertelli,
Effects of AMP and cyclic AMP on the mechanical and electrical activity of isolated mammalian atria.
1972,
Pubmed
Burnstock,
Purinergic nerves.
1972,
Pubmed
Busselen,
The effect of sodium, calcium and metabolic inhibitors on calcium efflux from goldfish heart ventricles.
1978,
Pubmed
Butler,
Myosin light chain phosphorylation does not modulate cross-bridge cycling rate in mouse skeletal muscle.
1983,
Pubmed
Caroni,
An ATP-dependent Ca2+-pumping system in dog heart sarcolemma.
1980,
Pubmed
Chapman,
Excitation-contraction coupling in cardiac muscle.
1979,
Pubmed
Crow,
Phosphorylation of myosin light chains in mouse fast-twitch muscle associated with reduced actomyosin turnover rate.
1982,
Pubmed
Ebashi,
Excitation-contraction coupling.
1976,
Pubmed
England,
Studies on the phosphorylation of the inhibitory subunit of troponin during modification of contraction in perfused rat heart.
1976,
Pubmed
England,
Correlation between contraction and phosphorylation of the inhibitory subunit of troponin in perfused rat heart.
1975,
Pubmed
Fabiato,
Calcium-induced release of calcium from the sarcoplasmic reticulum of skinned cells from adult human, dog, cat, rabbit, rat, and frog hearts and from fetal and new-born rat ventricles.
1978,
Pubmed
Fabiato,
Calcium and cardiac excitation-contraction coupling.
1979,
Pubmed
Flitney,
Inotropic responses of the frog ventricle to adenosine triphosphate and related changes in endogenous cyclic nucleotides.
1980,
Pubmed
Fozzard,
Heart: excitation-contraction coupling.
1977,
Pubmed
Frearson,
Changes in phosphorylation of P light chain of myosin in perfused rabbit heart.
,
Pubmed
Giles,
Changes in membrane currents in bullfrog atrium produced by acetylcholine.
1976,
Pubmed
Goto,
An analysis of the action of ATP and related compounds on membrane current and tension components in bullfrog atrial muscle.
1977,
Pubmed
Goto,
Tension fall after contraction of bullfrog atrial muscle examined with the voltage clamp technique.
1972,
Pubmed
HOLLANDER,
Effects of adenine nucleotides on the contractility and membrane potentials of rat atrium.
1957,
Pubmed
Hartzell,
Effects of cholinergic and adrenergic agonists on phosphorylation of a 165,000-dalton myofibrillar protein in intact cardiac muscle.
1982,
Pubmed
Hartzell,
Adenosine receptors in frog sinus venosus: slow inhibitory potentials produced by adenine compounds and acetylcholine.
1979,
Pubmed
Jeacocke,
Phosphorylation of a myofibrillar protein of Mr 150 000 in perfused rat heart, and the tentative indentification of this as C-protein.
1980,
Pubmed
Jundt,
The effect of substances releasing intracellular calcium ions on sodium-dependent calcium efflux from guinea-pig auricles.
1975,
Pubmed
Katz,
Role of the contractile proteins and sarcoplasmic reticulum in the response of the heart to catecholamines: an historical review.
1979,
Pubmed
Kavaler,
Electromechanical time course in frog ventricle: manipulation of calcium level during voltage clamp.
1974,
Pubmed
Kitazawa,
Physiological significance of Ca uptake by mitochondria in the heart in comparison with that by cardiac sarcoplasmic reticulum.
1976,
Pubmed
Kranias,
Phosphorylation of troponin I and phospholamban during catecholamine stimulation of rabbit heart.
1982,
Pubmed
Kranias,
Mechanism of the stimulation of calcium ion dependent adenosine triphosphatase of cardiac sarcoplasmic reticulum by adenosine 3',5'-monophosphate dependent protein kinase.
1980,
Pubmed
Langer,
Sodium-calcium exchange in the heart.
1982,
Pubmed
Langer,
Heart: excitation-contraction coupling.
1973,
Pubmed
Lindemann,
beta-Adrenergic stimulation of phospholamban phosphorylation and Ca2+-ATPase activity in guinea pig ventricles.
1983,
Pubmed
Mannherz,
Proteins of contractile systems.
1976,
Pubmed
Manning,
Protein phosphorylation: quantitative analysis in vivo and in intact cell systems.
1980,
Pubmed
McClellan,
Cyclic nucleotide regulation of the contractile proteins in mammalian cardiac muscle.
1980,
Pubmed
Meinertz,
Influence of cyclization and acyl substitution on the inotropic effects of adenine nucleotides.
1973,
Pubmed
Moos,
Interaction of C-protein with myosin, myosin rod and light meromyosin.
1975,
Pubmed
Moos,
The binding of skeletal muscle C-protein to F-actin, and its relation to the interaction of actin with myosin subfragment-1.
1978,
Pubmed
Moos,
Fluorescence microscope study of the binding of added C protein to skeletal muscle myofibrils.
1981,
Pubmed
Moos,
Effect of C-protein on actomyosin ATPase.
1980,
Pubmed
Mope,
Calcium sensitivity of the contractile system and phosphorylation of troponin in hyperpermeable cardiac cells.
1980,
Pubmed
Morad,
Excitation-concentration coupling in frog ventricle: evidence from voltage clamp studies.
1971,
Pubmed
Morad,
Rapid photochemical inactivation of Ca2+-antagonists shows that Ca2+ entry directly activates contraction in frog heart.
,
Pubmed
Nargeot,
Analysis of the negative inotropic effect of acetylcholine on frog atrial fibres.
1981,
Pubmed
Nargeot,
Time course of the increase in the myocardial slow inward current after a photochemically generated concentration jump of intracellular cAMP.
1983,
Pubmed
Offer,
A new protein of the thick filaments of vertebrate skeletal myofibrils. Extractions, purification and characterization.
1973,
Pubmed
Osterrieder,
Injection of subunits of cyclic AMP-dependent protein kinase into cardiac myocytes modulates Ca2+ current.
1982,
Pubmed
Page,
Structures of physiological interest in the frog heart ventricle.
1972,
Pubmed
Page,
Sterological measurements of cardiac ultrastructures implicated in excitation-contraction coupling.
1971,
Pubmed
Peachey,
The sarcoplasmic reticulum and transverse tubules of the frog's sartorius.
1965,
Pubmed
Reuter,
Localization of beta adrenergic receptors, and effects of noradrenaline and cyclic nucleotides on action potentials, ionic currents and tension in mammalian cardiac muscle.
1974,
Pubmed
Reuter,
Properties of two inward membrane currents in the heart.
1979,
Pubmed
Reuter,
Divalent cations as charge carriers in excitable membranes.
1973,
Pubmed
Reuter,
Calcium channel modulation by neurotransmitters, enzymes and drugs.
,
Pubmed
Reuter,
Exchange of calcium ions in the mammalian myocardium. Mechanisms and physiological significance.
1974,
Pubmed
Reuter,
The regulation of the calcium conductance of cardiac muscle by adrenaline.
1977,
Pubmed
Reuter,
Properties of single calcium channels in cardiac cell culture.
1982,
Pubmed
Robertson,
The effect of troponin I phosphorylation on the Ca2+-binding properties of the Ca2+-regulatory site of bovine cardiac troponin.
1982,
Pubmed
Robertson,
The time-course of Ca2+ exchange with calmodulin, troponin, parvalbumin, and myosin in response to transient increases in Ca2+.
1981,
Pubmed
Roulet,
The dependence of twitch relaxation on sodium ions and on internal Ca2+ stores in voltage clamped frog atrial fibres.
1979,
Pubmed
Shigekawa,
Calcium transport ATPase of canine cardiac sarcoplasmic reticulum. A comparison with that of rabbit fast skeletal muscle sarcoplasmic reticulum.
1976,
Pubmed
Solaro,
Estimating the functional capabilities of sarcoplasmic reticulum in cardiac muscle. Calcium binding.
1974,
Pubmed
Sommer,
Cardiac muscle. A comparative study of Purkinje fibers and ventricular fibers.
1968,
Pubmed
Sommer,
Cardiac muscle. A comparative ultrastructural study with special reference to frog and chicken hearts.
1969,
Pubmed
Staley,
The ultrastructure of frog ventricular cardiac muscle and its relationship to mechanism of excitation-contraction coupling.
1968,
Pubmed
Stull,
Phosphorylation of contractile proteins in relation to muscle function.
1980,
Pubmed
Tada,
The stimulation of calcium transport in cardiac sarcoplasmic reticulum by adenosine 3':5'-monophosphate-dependent protein kinase.
1974,
Pubmed
Tada,
Transient state kinetic studies of Ca2+-dependent ATPase and calcium transport by cardiac sarcoplasmic reticulum. Effect of cyclic AMP-dependent protein kinase-catalyzed phosphorylation of phospholamban.
1980,
Pubmed
Tada,
Phosphorylation of the sarcoplasmic reticulum and sarcolemma.
1982,
Pubmed
Trautwein,
The effect of intracellular cyclic nucleotides and calcium on the action potential and acetylcholine response of isolated cardiac cells.
1982,
Pubmed
Tsien,
Cyclic AMP and contractile activity in heart.
1977,
Pubmed
Tsien,
Adrenaline-like effects of intracellular iontophoresis of cyclic AMP in cardiac Purkinje fibres.
1973,
Pubmed
Van Winkle,
Ions and inotropy.
1976,
Pubmed
WEIDMANN,
Effect of increasing the calcium concentration during a single heart-beat.
1959,
Pubmed
Weisberg,
Regulation of calcium sensitivity in perforated mammalian cardiac cells.
1983,
Pubmed
Will,
A quench-flow kinetic investigation of calcium ion accumulation by isolated cardiac sarcoplasmic reticulum. Dependence of initial velocity on free calcium ion concentration and influence of preincubation with a protein kinase, MgATP, and cyclic AMP.
1976,
Pubmed
Winegrad,
Regulation of cardiac contractile proteins by phosphorylation.
1983,
Pubmed
Winegrad,
Calcium release from cardiac sarcoplasmic reticulum.
1982,
Pubmed
Wollenberger,
Protein kinase-catalyzed membrane phosphorylation and its possible relationship to the role of calcium in the adrenergic regulation of cardiac contraction.
,
Pubmed
Wray,
Cyclic AMP stimulation of membrane phosphorylation and Ca2+-activated, Mg2+-dependent ATPase in cardiac sarcoplasmic reticulum.
1977,
Pubmed
Yamamoto,
The C-proteins of rabbit red, white, and cardiac muscles.
1983,
Pubmed