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Inhibition of eukaryotic tRNA transcription by potential Z-DNA sequences.
Santoro C
,
Costanzo F
,
Ciliberto G
.
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The effect of d(CA/TG)n DNA segments on tRNA transcription has been examined. Alternating purine-pyrimidine tracts were cloned at a long distance from, adjacent to, or within the coding sequence of a tRNAPro gene from Caenorhabditis elegans and shown to be able to assume the A-DNA conformation in vitro in physiological salt concentrations. The transcriptional level of these constructs was compared to that of normal tDNAPro by micro-injection into Xenopus laevis oocytes. Our results show a strong inhibitory effect by potential Z-DNA sequences only when these are placed in the flanking regions of the gene or when they are located between the elements (Box A and Box B) of the split promoter. Transcription was studied in parallel with supercoiled and linear DNA molecules carrying a d(CA/TG) stretch 124-bp long in front of the tRNAPro gene. The results show the same level of inhibition of Po/III transcription regardless of the topological status of the injected DNA.
Castagnoli,
Processing of eukaryotic tRNA precursors: secondary structure of the precursor specific sequences affects the rate but not the accuracy of processing reactions.
1982, Pubmed
Castagnoli,
Processing of eukaryotic tRNA precursors: secondary structure of the precursor specific sequences affects the rate but not the accuracy of processing reactions.
1982,
Pubmed
Ciliberto,
Transcription by RNA polymerase III.
1983,
Pubmed
Ciliberto,
Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
1982,
Pubmed
,
Xenbase
Ciliberto,
Relationship between the two components of the split promoter of eukaryotic tRNA genes.
1982,
Pubmed
,
Xenbase
Cortese,
Cloning of nematode tRNA genes and their expression in the frog oocyte.
1978,
Pubmed
,
Xenbase
Cortese,
Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
1980,
Pubmed
,
Xenbase
DeFranco,
Two control regions for eukaryotic tRNA gene transcription.
1980,
Pubmed
,
Xenbase
Fried,
CAP binding to B and Z forms of DNA.
1983,
Pubmed
Galli,
Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
1981,
Pubmed
,
Xenbase
Hamada,
Potential Z-DNA forming sequences are highly dispersed in the human genome.
1982,
Pubmed
Hamada,
A novel repeated element with Z-DNA-forming potential is widely found in evolutionarily diverse eukaryotic genomes.
1982,
Pubmed
Haniford,
Facile transition of poly[d(TG) x d(CA)] into a left-handed helix in physiological conditions.
1983,
Pubmed
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Hipskind,
5'-flanking sequences that inhibit in vitro transcription of a xenopus laevis tRNA gene.
1983,
Pubmed
,
Xenbase
Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase
Keller,
Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.
1975,
Pubmed
Klemenz,
Specific interactions of Saccharomyces cerevisiae proteins with a promoter region of eukaryotic tRNA genes.
1982,
Pubmed
Kłysik,
Left-handed DNA in restriction fragments and a recombinant plasmid.
1981,
Pubmed
Larsen,
An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
1982,
Pubmed
Nickol,
Effect of the B--Z transition in poly(dG-m5dC) . poly(dG-m5dC) on nucleosome formation.
1982,
Pubmed
Nishioka,
Organization and complete sequence of identical embryonic and plasmacytoma kappa V-region genes.
1980,
Pubmed
Nordheim,
Negatively supercoiled simian virus 40 DNA contains Z-DNA segments within transcriptional enhancer sequences.
,
Pubmed
Nordheim,
Isolation of Drosophila proteins that bind selectively to left-handed Z-DNA.
1982,
Pubmed
Nordheim,
Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.
1982,
Pubmed
Nordheim,
The sequence (dC-dA)n X (dG-dT)n forms left-handed Z-DNA in negatively supercoiled plasmids.
1983,
Pubmed
Panayotatos,
Cruciform structures in supercoiled DNA.
1981,
Pubmed
Razin,
DNA methylation and gene function.
1980,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Santoro,
Stretches of alternating poly(T-dG), with the capacity to form Z-DNA, are present in human liver transcripts.
1983,
Pubmed
Schaack,
Transcription of eukaryotic tRNA genes in vitro. II. Formation of stable complexes.
1983,
Pubmed
Sharp,
Internal control regions for transcription of eukaryotic tRNA genes.
1981,
Pubmed
,
Xenbase
Singleton,
Left-handed Z-DNA is induced by supercoiling in physiological ionic conditions.
1982,
Pubmed
Singleton,
The facile generation of covalently closed, circular DNAs with defined negative superhelical densities.
1982,
Pubmed
Slightom,
Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
1980,
Pubmed
Sutcliffe,
Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
1979,
Pubmed
Traboni,
Mutations in Box B of the promoter of a eucaryotic tRNAPro gene affect rate of transcription, processing, and stability of the transcripts.
1984,
Pubmed
,
Xenbase
Traboni,
A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
1982,
Pubmed
,
Xenbase
Vasicek,
Nucleotide sequence of the human parathyroid hormone gene.
1983,
Pubmed
Wang,
Molecular structure of a left-handed double helical DNA fragment at atomic resolution.
1979,
Pubmed
Weil,
Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
1979,
Pubmed
Weintraub,
A dominant role for DNA secondary structure in forming hypersensitive structures in chromatin.
1983,
Pubmed
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase