Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Virol
1984 Aug 01;512:437-44. doi: 10.1128/JVI.51.2.437-444.1984.
Show Gene links
Show Anatomy links
Essential nucleotides in the polyomavirus origin region.
Triezenberg SJ
,
Folk WR
.
???displayArticle.abstract???
We have isolated a series of point mutants in the polyomavirus origin-intergenic control region by using a procedure which exploits the single-stranded nature of DNAs cloned into M13 phage both for the generation of mutants and for their analysis by DNA sequencing. In this report we describe the effects of cytosine X guanine----thymine X adenine base-pair substitutions in the polyomavirus origin region upon replication in mouse 3T6 cells of the M13-polyomavirus constructs. Our results indicate that sequences near the center of a 34-base-pair sequence with dyad symmetry are important for replication, whereas specific nucleotides near the ends of the dyad symmetry are not important. Furthermore, a putative large T antigen-binding site at nucleotides 60 to 80 can be mutated without affecting replication function as measured in this assay.
Bendig,
Regulatory mutants of polyoma virus defective in DNA replication and the synthesis of early proteins.
1980, Pubmed
Bendig,
Regulatory mutants of polyoma virus defective in DNA replication and the synthesis of early proteins.
1980,
Pubmed
DeLucia,
Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication.
1983,
Pubmed
Ding,
Mutant din-21, a variant of polyoma virus containing a mouse DNA sequence in the viral genome.
1982,
Pubmed
Everett,
A rapid and efficient method for region- and strand-specific mutagenesis of cloned DNA.
1982,
Pubmed
Folk,
A detailed mutational analysis of the eucaryotic tRNAmet1 gene promoter.
1983,
Pubmed
,
Xenbase
Francke,
Polyoma gene function required for viral DNA synthesis.
1973,
Pubmed
Friedmann,
The nucleotide sequence and genome organization of the polyoma early region: extensive nucleotide and amino acid homology with SV40.
1979,
Pubmed
Frost,
Mapping temperature-sensitive and host-range mutations of adenovirus type 5 by marker rescue.
1978,
Pubmed
Gaudray,
The high affinity binding site on polyoma virus DNA for the viral large-T protein.
1981,
Pubmed
Gluzman,
Origin-defective mutants of SV40.
1980,
Pubmed
Graham,
A new technique for the assay of infectivity of human adenovirus 5 DNA.
1973,
Pubmed
Hay,
Initiation of SV40 DNA replication in vivo: location and structure of 5' ends of DNA synthesized in the ori region.
1982,
Pubmed
Hirt,
Selective extraction of polyoma DNA from infected mouse cell cultures.
1967,
Pubmed
Jones,
Essential contact residues within SV40 large T antigen binding sites I and II identified by alkylation-interference.
1984,
Pubmed
Kamen,
5' termini of polyoma virus early region transcripts synthesized in vivo by wild-type virus and viable deletion mutants.
1982,
Pubmed
Katinka,
DNA replication origin of polyoma virus: early proximal boundary.
1983,
Pubmed
Lusky,
Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
1981,
Pubmed
Luthman,
Non-contiguous segments of the polyoma genome required in cis for DNA replication.
1982,
Pubmed
Margolskee,
Simian virus 40 mutant T antigens with relaxed specificity for the nucleotide sequence at the viral DNA origin of replication.
1984,
Pubmed
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Muller,
Polyomavirus origin for DNA replication comprises multiple genetic elements.
1983,
Pubmed
Myers,
Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication.
1980,
Pubmed
O'Hare,
Replication of polyoma plasmid recombinants in mouse cells.
1981,
Pubmed
Pomerantz,
Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome.
1983,
Pubmed
Rio,
Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.
1980,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Shortle,
Regulatory mutants of simian virus 40: constructed mutants with base substitutions at the origin of DNA replication.
1979,
Pubmed
Shortle,
Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin.
1979,
Pubmed
Shortle,
Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.
1978,
Pubmed
Soeda,
Coding potential and regulatory signals of the polyoma virus genome.
1980,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Tegtmeyer,
Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
1983,
Pubmed
Tjian,
Protein-DNA interactions at the origin of simian virus 40 DNA replication.
1979,
Pubmed
Triezenberg,
Structure of the Syrian hamster ribosomal DNA repeat and identification of homologous and nonhomologous regions shared by human and hamster ribosomal DNAs.
1982,
Pubmed
Tyndall,
A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication.
1981,
Pubmed
van der Eb,
Assay of transforming activity of tumor virus DNA.
1980,
Pubmed
