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A transfer RNAArg gene of Pelargonium chloroplasts, but not a 5S RNA gene, is efficiently transcribed after injection into Xenopus oocyte nuclei.
Hellmund D
,
Metzlaff M
,
Serfling E
.
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We present the primary structure of a chloroplast tRNAArgACG gene of the plant, Pelargonium zonale, and its faithful expression in Xenopus oocyte nuclei. This tRNAArg gene is located 250 bp downstream of a 5S RNA gene within a cloned 5kb long ribosomal DNA segment (Fig. 1). The Pelargonium tRNAArg gene shares 97% and 86% sequence homology with tRNAArgACG genes of Spirodela oligorhiza and Euglena gracilis chloroplasts, respectively, and also extensive homology (70%) with the corresponding gene of E. coli. It lacks an intervening sequence and, like eukaryotic tRNA genes, does not code for the 3' terminal CCA nucleotides. Moreover, the chloroplast tRNAArg gene carries all the sequence elements essential for transcription by vertebrate RNA polymerase III since it is efficiently expressed in Xenopus oocyte nuclei, even in the presence of 1 microgram/ml alpha-amanitin. In Xenopus oocyte nuclei, no transcripts of the chloroplast 5S RNA gene were detected.
Bartnik,
Mitochondrial transfer RNA genes from fungi (Aspergillus nidulans) and plants (Lupinus luteus) are transcribed in Xenopus laevis oocyte nuclei.
1983, Pubmed,
Xenbase
Bartnik,
Mitochondrial transfer RNA genes from fungi (Aspergillus nidulans) and plants (Lupinus luteus) are transcribed in Xenopus laevis oocyte nuclei.
1983,
Pubmed
,
Xenbase
Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase
Ciliberto,
Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
1982,
Pubmed
,
Xenbase
Ciliberto,
Relationship between the two components of the split promoter of eukaryotic tRNA genes.
1982,
Pubmed
,
Xenbase
Ciliberto,
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
1983,
Pubmed
,
Xenbase
Delihas,
The nucleotide sequence of the chloroplast 5S ribosomal RNA from spinach.
1981,
Pubmed
Dente,
A prokaryotic tRNATyr gene, inactive in Xenopus laevis oocytes, is activated by recombination with an eukaryotic tRNAPro gene.
1982,
Pubmed
,
Xenbase
Edwards,
The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA.
1981,
Pubmed
Folk,
Some bacterial tRNA genes are transcribed by eukaryotic RNA polymerase III.
1982,
Pubmed
,
Xenbase
Folk,
A detailed mutational analysis of the eucaryotic tRNAmet1 gene promoter.
1983,
Pubmed
,
Xenbase
Galli,
Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
1981,
Pubmed
,
Xenbase
Gauss,
Compilation of tRNA sequences.
1983,
Pubmed
Gauss,
Compilation of sequences of tRNA genes.
1983,
Pubmed
Gruissem,
Transcription of E. coli and Euglena chloroplast tRNA gene clusters and processing of polycistronic transcripts in a HeLa cell-free system.
1982,
Pubmed
Gruissem,
Biosynthesis of chloroplast transfer RNA in a spinach chloroplast transcription system.
1983,
Pubmed
Hartley,
The synthesis of chloroplast high-molecular-weight ribosomal ribonucleic acid in spinach.
1979,
Pubmed
Haumont,
Enzymatic conversion of adenosine to inosine in the wobble position of yeast tRNAAsp: the dependence on the anticodon sequence.
1984,
Pubmed
,
Xenbase
Herrmann,
Size, conformation and purity of chloroplast DNA of some higher plants.
1975,
Pubmed
Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase
Kato,
The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts.
1981,
Pubmed
Keus,
The nucleotide sequences of the genes coding for tRNAArgUCU, tRNAArgACG and tRNAAsnGUU on Spirodela oligorhiza chloroplast DNA.
1984,
Pubmed
Koch,
Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.
1981,
Pubmed
Kressmann,
Transcription of xenopus tDNAmet1 and sea urchin histone DNA injected into the Xenopus oocyte nucleus.
1978,
Pubmed
,
Xenbase
Mao,
The 5S RNA genes of Schizosaccharomyces pombe.
1982,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase
Mertz,
Purified DNAs are transcribed after microinjection into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Murao,
Primary sequence of arginine transfer RNA from Escherichia coli.
1972,
Pubmed
Nishikura,
RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.
1981,
Pubmed
,
Xenbase
Orozco,
Euglena gracilis chloroplast transfer RNA transcription units. II. Nucleotide sequence analysis of a tRNAVal-tRNAAsn-tRNAArg-tRNALeu gene cluster.
1982,
Pubmed
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Rosenberg,
Regulatory sequences involved in the promotion and termination of RNA transcription.
1979,
Pubmed
Siebenlist,
E. coli RNA polymerase interacts homologously with two different promoters.
1980,
Pubmed
Smith,
A simple method for DNA restriction site mapping.
1976,
Pubmed
Takaiwa,
Nucleotide sequence of the 16S - 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA.
1982,
Pubmed
Traboni,
A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
1982,
Pubmed
,
Xenbase
Wildeman,
Nucleotide sequence of wheat chloroplastid 4.5 S ribonucleic acid. Sequence homologies in 4.5 S RNA species.
1980,
Pubmed