Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Membr Biol
2004 Jun 01;1993:135-41. doi: 10.1007/s00232-004-0684-9.
Show Gene links
Show Anatomy links
Transient permeability leak of nuclear envelope induced by aldosterone.
Buchholz I
,
Enss K
,
Schafer C
,
Schlune A
,
Shahin V
,
Oberleithner H
.
???displayArticle.abstract???
The mineralocorticoid hormone aldosterone controls fluid and electrolyte transport in target cells of the kidney and the cardiovascular system. Classic genomic aldosterone action involves the activation of cytosolic mineralocorticoid receptors and translocation into the cell nucleus where specific transcription processes are initiated. A key barrier of the intracellular signalling pathway is the nuclear envelope, which physically separates the nucleoplasm from the cytoplasm. It was shown recently that aldosterone changes ion conductivity of the nuclear envelope mediated by nuclear pore complexes. The latter are supramolecular nanomachines responsible for import and export of inorganic ions and macromolecules. The aim of the present study was to test whether aldosterone changes the macromolecule permeability of the nuclear envelope. Aldosterone-responsive Xenopus laevis oocytes were used as a model system. We isolated the cell nuclei at defined times after hormone injection. By means of confocal fluorescence microscopy and fluorescence-labelled dextrans we evaluated passive macromolecule import and export in isolated nuclei. 10 minutes after aldosterone injection nuclear envelope permeability of 10 kD dextran was found sharply increased. At the same time cell nuclei were found swollen by about 28%. Changes in nuclear volume and nuclear envelope permeability lasted 5 to 15 minutes and could be inhibited by the mineralocorticoid receptor blocker spironolactone. We conclude that aldosterone transiently changes the barrier function of the nuclear envelope. This short-lasting permeability change signals the start of a sustained transcription process that follows in response to steroids.
Daneholt,
A look at messenger RNP moving through the nuclear pore.
1997, Pubmed
Daneholt,
A look at messenger RNP moving through the nuclear pore.
1997,
Pubmed
Danker,
Electrophoretic plugging of nuclear pores by using the nuclear hourglass technique.
2001,
Pubmed
,
Xenbase
Danker,
Nuclear hourglass technique: an approach that detects electrically open nuclear pores in Xenopus laevis oocyte.
1999,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Enss,
Passive transport of macromolecules through Xenopus laevis nuclear envelope.
2003,
Pubmed
,
Xenbase
Golden,
Accumulation of individual pA+ RNAs during oogenesis of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Greber,
Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein.
1992,
Pubmed
Harvey,
Nongenomic effects of aldosterone on Ca2+ in M-1 cortical collecting duct cells.
2000,
Pubmed
Hinshaw,
Architecture and design of the nuclear pore complex.
1992,
Pubmed
,
Xenbase
Jäggi,
Quantitative topographical analysis of nuclear pore complex function using scanning force microscopy.
2003,
Pubmed
,
Xenbase
Kroeger,
[Potential difference and puff patterns. Electrophysiologic and cytologic studies of the salivary glands of Chironomus thummi].
1966,
Pubmed
Loewenstein,
Permeability of a nuclear membrane: changes during normal development and changes induced by growth hormone.
1965,
Pubmed
Mazzanti,
Electrical dimension of the nuclear envelope.
2001,
Pubmed
Mazzanti,
ATP-dependent ionic permeability on nuclear envelope in in situ nuclei of Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Oberleithner,
Route of steroid-activated macromolecules through nuclear pores imaged with atomic force microscopy.
2003,
Pubmed
,
Xenbase
Oberleithner,
Aldosterone activates Na+/H+ exchange and raises cytoplasmic pH in target cells of the amphibian kidney.
1987,
Pubmed
Oberleithner,
Imaging nuclear pores of aldosterone-sensitive kidney cells by atomic force microscopy.
1994,
Pubmed
Paine,
Nuclear envelope permeability.
1975,
Pubmed
Panté,
The nuclear pore complex.
1993,
Pubmed
Perez-Terzic,
Conformational states of the nuclear pore complex induced by depletion of nuclear Ca2+ stores.
1996,
Pubmed
,
Xenbase
Peters,
Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis.
1984,
Pubmed
Reichelt,
Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components.
1990,
Pubmed
,
Xenbase
Schäfer,
Aldosterone signaling pathway across the nuclear envelope.
2002,
Pubmed
,
Xenbase
Schäfer,
Intracellular calcium: a prerequisite for aldosterone action.
2003,
Pubmed
,
Xenbase
Shahin,
Evidence for Ca2+- and ATP-sensitive peripheral channels in nuclear pore complexes.
2001,
Pubmed
,
Xenbase
Stehno-Bittel,
Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store.
1995,
Pubmed
,
Xenbase
WATSON,
The nuclear envelope; its structure and relation to cytoplasmic membranes.
1955,
Pubmed
Wang,
Conformational changes of the in situ nuclear pore complex.
1999,
Pubmed
,
Xenbase
Wünsch,
20-OH-ecdysone swells nuclear volume by alkalinization in salivary glands of Drosophila melanogaster.
1993,
Pubmed