Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
DNase I footprinting shows three protected regions in the promoter of the rRNA genes of Xenopus laevis.
Dunaway M
,
Reeder RH
.
???displayArticle.abstract???
Extracts prepared from Xenopus laevis oocytes contain a protein(s) which specifically protects three discrete regions of the RNA polymerase I promoter from digestion by DNase I. Protected region I, from nucleotide +15 to nucleotide -10, spans the site of transcription initiation. Protected region II extends from nucleotide -70 to nucleotide -100 relative to initiation, falling within a 42-base-pair sequence which is homologous to the 60/81-base-pair repeated elements which occur outside of the promoter in the spacer. Protected region III is upstream of region II, from nucleotide -120 to nucleotide -140. All three regions correlate with sequences known from deletion studies to be important for promoter function. Deletion mutants which retain either region I or regions II and III together footprint normally. Deletion of region III, however, reduces but does not eliminate footprinting on region II, suggesting either that one protein binds to both regions or that the proteins which bind to these sites interact with each other.
Aiba,
Autoregulation of the Escherichia coli crp gene: CRP is a transcriptional repressor for its own gene.
1983, Pubmed
Aiba,
Autoregulation of the Escherichia coli crp gene: CRP is a transcriptional repressor for its own gene.
1983,
Pubmed
Bach,
Sequence organization of the spacer in the ribosomal genes of Xenopus clivii and Xenopus borealis.
1981,
Pubmed
,
Xenbase
Bakken,
Mapping of transcription initiation and termination signals on Xenopus laevis ribosomal DNA.
1982,
Pubmed
,
Xenbase
Boseley,
Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Busby,
Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.
1983,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Dynan,
The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
1983,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Fuhrman,
HeLa cell RNA polymerase III transcription factors. Functional characterization of a fraction identified by its activity in a second template rescue assay.
1984,
Pubmed
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Hofstetter,
A split promoter for a eucaryotic tRNA gene.
1981,
Pubmed
,
Xenbase
Labhart,
Enhancer-like properties of the 60/81 bp elements in the ribosomal gene spacer of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McKnight,
Transcriptional control signals of a eukaryotic protein-coding gene.
1982,
Pubmed
McStay,
The origin of the rRNA precursor from Xenopus borealis, analysed in vivo and in vitro.
1983,
Pubmed
,
Xenbase
Miller,
Template structural requirements for transcription in vivo by RNA polymerase II.
1982,
Pubmed
,
Xenbase
Moss,
Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.
1982,
Pubmed
,
Xenbase
Moss,
A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.
,
Pubmed
,
Xenbase
Parker,
A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity.
1984,
Pubmed
Parker,
A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
1984,
Pubmed
Pruitt,
Effect of topological constraint on transcription of ribosomal DNA in Xenopus oocytes. Comparison of plasmid and endogenous genes.
1984,
Pubmed
,
Xenbase
Reeder,
Spacer regulation of Xenopus ribosomal gene transcription: competition in oocytes.
1983,
Pubmed
,
Xenbase
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Sanger,
The use of thin acrylamide gels for DNA sequencing.
1978,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Sollner-Webb,
Nested control regions promote Xenopus ribosomal RNA synthesis by RNA polymerase I.
1983,
Pubmed
,
Xenbase
Wilkinson,
Dinucleotide primers facilitate convenient identification of the mouse ribosomal DNA transcription initiation site. A general method for analysis of transcription by RNA polymerases I and III.
1983,
Pubmed
