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Soreq H
,
Zevin-Sonkin D
,
Avni A
,
Hall LM
,
Spierer P
.
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The Ace locus of the Drosophila genome controls biosynthesis of the neurotransmitter-hydrolyzing enzyme acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7). We injected the mRNA species hybridizing with DNA fragments from this region into Xenopus oocytes, in which acetylcholinesterase mRNA is translated into active acetylcholinesterase. A 2.0-kilobase (kb) fragment of DNA from this region selectively hybridizes with Drosophila mRNA capable of inducing the biosynthesis of acetylcholinesterase in oocytes. This Drosophila DNA fragment cross-hybridized with human brain poly(A)+ RNA. We therefore used this DNA fragment as a probe for homologous sequence(s) in a human genomic DNA library and thus selected a 13.5-kb human DNA segment. DNA blot-hybridization revealed that a 2.6-kb fragment of this human DNA segment hybridizes with the Drosophila 2.0-kb DNA fragment. Both Drosophila and human fragments hybridized with a human brain mRNA species of about 7.0-kb that was barely detectable in the acetylcholinesterase-deficient HEp carcinoma. A fraction containing mRNA of similar size, extracted from human brain, induced acetylcholinesterase biosynthesis in oocytes. The human DNA fragment also was used in hybridization-selection experiments. In oocytes, hybrid-selected human brain mRNA induced acetylcholinesterase activity that was completely inhibited by 1,5-bis[4-allyldimethylammonium)phenyl]pentan-3-one dibromide but not by tetraisopropyl pyrophosphamide, a differential response to these inhibitors characteristic of "true" human brainacetylcholinesterase. These findings strongly suggest that both the Drosophila and the human DNA fragments are directly involved in controlling acetylcholinesterase biosynthesis.
AUSTIN,
Two selective inhibitors of cholinesterase.
1953, Pubmed
AUSTIN,
Two selective inhibitors of cholinesterase.
1953,
Pubmed
Atack,
Molecular forms of acetylcholinesterase in senile dementia of Alzheimer type: selective loss of the intermediate (10S) form.
1983,
Pubmed
Bender,
Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster.
1983,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Birnboim,
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
1979,
Pubmed
Burmeister,
Production of EGF-containing polypeptides in Xenopus oocytes microinjected with submaxillary gland mRNA.
1984,
Pubmed
,
Xenbase
Cherbas,
Induction of acetylcholinesterase activity by beta-ecdysone in a Drosophila cell line.
1977,
Pubmed
Coyle,
Alzheimer's disease: a disorder of cortical cholinergic innervation.
1983,
Pubmed
Drews,
Cholinesterase in embryonic development.
1975,
Pubmed
Dudai,
Purification by affinity chromatography of the molecular forms of acetylcholinesterase present in fresh electric-organ tissue of electric eel.
1972,
Pubmed
Hall,
Transcripts, genes and bands in 315,000 base-pairs of Drosophila DNA.
1983,
Pubmed
Hall,
Genetics of acetylcholinesterase in Drosophila melanogaster.
1976,
Pubmed
Hilliker,
Cytogenetic analysis of the chromosomal region immediately adjacent to the rosy locus in Drosophila melanogaster.
1980,
Pubmed
Johnson,
A rapid, simple radiometric assay for cholinesterase, suitable for multiple determinations.
1975,
Pubmed
Layer,
Comparative localization of acetylcholinesterase and pseudocholinesterase during morphogenesis of the chicken brain.
1983,
Pubmed
Leong,
Energy-metabolizing enzymes in brain regions of adult and aging rats.
1981,
Pubmed
Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed
Massoulié,
The molecular forms of cholinesterase and acetylcholinesterase in vertebrates.
1982,
Pubmed
McDonell,
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
1977,
Pubmed
Poncz,
Construction of human gene libraries from small amounts of peripheral blood: analysis of beta-like globin genes.
1982,
Pubmed
Razon,
Characterization of activities and forms of cholinesterases in human primary brain tumors.
1984,
Pubmed
Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed
Rosenberry,
Structure of human erythrocyte acetylcholinesterase. Characterization of intersubunit disulfide bonding and detergent interaction.
1984,
Pubmed
Shilo,
DNA sequences homologous to vertebrate oncogenes are conserved in Drosophila melanogaster.
1981,
Pubmed
Soreq,
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
1982,
Pubmed
,
Xenbase
Soreq,
Expression of cholinesterase gene(s) in human brain tissues: translational evidence for multiple mRNA species.
1984,
Pubmed
,
Xenbase
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Spierer,
Molecular mapping of genetic and chromomeric units in Drosophila melanogaster.
1983,
Pubmed
Spokes,
Neurochemical alterations in Huntington's chorea: a study of post-mortem brain tissue.
1980,
Pubmed
Sørensen,
An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties.
1982,
Pubmed
Vigny,
"Nonspecific" cholinesterase and acetylcholinesterase in rat tissues: molecular forms, structural and catalytic properties, and significance of the two enzyme systems.
1978,
Pubmed
Zingde,
Molecular properties of Drosophila acetylcholinesterase.
1983,
Pubmed
