Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Human 2-5A synthetase: characterization of a novel cDNA and corresponding gene structure.
Saunders ME
,
Gewert DR
,
Tugwell ME
,
McMahon M
,
Williams BR
.
???displayArticle.abstract???
The enzyme 2-5A synthetase is induced in cultured cells in response to interferon (IFN) treatment. A lambda gt10 cDNA library of mRNA from IFN-induced Daudi lymphoblastoid cells was screened with oligonucleotide probes. Several overlapping cDNAs were isolated and shown to be derived from the human synthetase gene using filter selection and oocyte microinjection assays. The nucleotide sequence of one of these, cDNA 8-2, extended the 2-5A synthetase sequence already described 72 bp in the 5' direction but was found to differ significantly in coding sequence at the 3' end. The longest cDNA isolated (6-2) was approximately 1.4 kb. By Northern hybridization analysis single mRNAs of 1.7 kb were detected in Daudi and T98G (glioblastoma) cells. However, in HeLa cells, four mRNAs ranging in size from 1.5 to 3.5 kb were found, one of which differed at the 3' end. Analysis of both phage and cosmid genomic clones and comparison with genomic DNA indicate that there is a single gene for 2-5A synthetase, comprising at least six exons and five introns, which can undergo a novel form of alternative RNA processing depending on cell type.
Benech,
3' end structure of the human (2'-5') oligo A synthetase gene: prediction of two distinct proteins with cell type-specific expression.
1985, Pubmed
Benech,
3' end structure of the human (2'-5') oligo A synthetase gene: prediction of two distinct proteins with cell type-specific expression.
1985,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Clemens,
Inhibition of cell-free protein synthesis by pppA2'p5'A2'p5'A: a novel oligonucleotide synthesized by interferon-treated L cell extracts.
1978,
Pubmed
De Maeyer,
Expression of a chemically synthesized human alpha 1 interferon gene.
1982,
Pubmed
Friedman,
Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells.
1984,
Pubmed
Gewert,
Inhibition of cell division by interferons: Changes in the transport and intracellular metabolism of thymidine in human lymphoblastoid (Daudi) cells.
1981,
Pubmed
Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
King,
Alternative RNA splicing of the murine alpha A-crystallin gene: protein-coding information within an intron.
1983,
Pubmed
Krause,
Regulation of ppp(A2'p)nA-dependent RNase levels during interferon treatment and cell differentiation.
1985,
Pubmed
Maniatis,
The isolation of structural genes from libraries of eucaryotic DNA.
1978,
Pubmed
Merlin,
Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.
1983,
Pubmed
Nabeshima,
Alternative transcription and two modes of splicing results in two myosin light chains from one gene.
,
Pubmed
Penn,
Interferon-induced 2-5A synthetase activity in human peripheral blood mononuclear cells after immunization with influenza virus and rubella virus vaccines.
1984,
Pubmed
Poustka,
Selective isolation of cosmid clones by homologous recombination in Escherichia coli.
1984,
Pubmed
Rosenfeld,
Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing.
,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Silverman,
The ppp(A2'p)nA and protein kinase systems in wild-type and interferon-resistant Daudi cells.
1982,
Pubmed
St Laurent,
Interferon action: two (2'-5')(A)n synthetases specified by distinct mRNAs in Ehrlich ascites tumor cells treated with interferon.
1983,
Pubmed
,
Xenbase
Wells,
An improved method for purifying 2',5'-oligoadenylate synthetases.
1984,
Pubmed
Williams,
Synthesis and breakdown of pppA2'p5'A2'p5'A and transient inhibiton of protein synthesis in extracts from interferon-treated and control cells.
1978,
Pubmed
Williams,
Natural occurrence of 2-5A in interferon-treated EMC virus-infected L cells.
1979,
Pubmed
Williams,
Assay of (2'-5')-oligo(A) synthesized in vitro and the analysis of naturally occurring (2'-5')-oligo(A) from intact cells.
1981,
Pubmed
Wreschner,
Interferon action--sequence specificity of the ppp(A2'p)nA-dependent ribonuclease.
1981,
Pubmed
