Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The localization of MC25, an antigen first detected on the surface of human neuroblastoma cells, was determined in cultured cells and tissues. Neuroblastoma cell lines (15/17) express the antigen on the surface and in the cytoplasm (scMC25+), whereas 156/160 cell lines derived from other normal and malignant human cell types are scMC25-. However, MC25 is found in the nucleus of scMC25- cells (nMC25+), presenting a discrete granular pattern. In scMC25+/nMC25- neuroblastoma lines, apparent antigen shifting from the cell surface/cytoplasm to the nucleus accompanies variant formation, which represents a transition in the neuronal differentiation program of these cells. Results of immunohistochemical studies with human tissues parallel the findings with cultured cells. Almost all cell types are scMC25-/nMC25+; basal cells of the epidermis are the only cells constitutively expressing cMC25; and a population of neurons are the only scMC25-/nMC25- cells. Alternative localization of MC25 to different cellular compartments and antigen shifting are reminiscent of the behavior of certain developmentally regulated antigens in Drosophila and Xenopus.
Ashihara,
Autoradiographic studies on cell proliferation and differentiation in the human epidermis in vivo.
1967, Pubmed
Ashihara,
Autoradiographic studies on cell proliferation and differentiation in the human epidermis in vivo.
1967,
Pubmed
Bernal,
In vitro and in vivo growth characteristics of two different cell populations in an established line of human neuroblastoma.
1983,
Pubmed
Biedler,
Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.
1973,
Pubmed
Biedler,
A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.
1976,
Pubmed
COONS,
Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit.
1955,
Pubmed
Casper,
Distinguishing characteristics of a new neuroblastoma cell line.
1983,
Pubmed
Dequin,
Proteins shifting from the cytoplasm into the nuclei during early embryogenesis of Drosophila melanogaster.
1984,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dippold,
Cell surface antigens of human malignant melanoma: definition of six antigenic systems with mouse monoclonal antibodies.
1980,
Pubmed
Dreyer,
Two-dimensional gel analysis of the fate of oocyte nuclear proteins in the development of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Greaves,
"Target" cells, cellular phenotypes, and lineage fidelity in human leukaemia.
1982,
Pubmed
Hsu,
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.
1981,
Pubmed
Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed
Lanford,
Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen.
1984,
Pubmed
Mattes,
Preparing monolayers of non-adherent mammalian cells.
1983,
Pubmed
Meyers,
Gene amplification-associated cytogenetic aberrations and protein changes in vincristine-resistant Chinese hamster, mouse, and human cells.
1985,
Pubmed
Penneys,
Location of proliferating cells in human epidermis.
1970,
Pubmed
Rettig,
High-molecular-weight glycoproteins of human teratocarcinoma defined by monoclonal antibodies to carbohydrate determinants.
1985,
Pubmed
Reynolds,
Selective toxicity of 6-hydroxydopamine and ascorbate for human neuroblastoma in vitro: a model for clearing marrow prior to autologous transplant.
1982,
Pubmed
Ross,
Neurotransmitter-synthesizing enzymes in 14 human neuroblastoma cell lines.
1981,
Pubmed
Ross,
Coordinate morphological and biochemical interconversion of human neuroblastoma cells.
1983,
Pubmed
Zeller,
Nucleocytoplasmic distribution of snRNPs and stockpiled snRNA-binding proteins during oogenesis and early development in Xenopus laevis.
1983,
Pubmed
,
Xenbase