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Genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family.
Nagao RT
,
Czarnecka E
,
Gurley WB
,
Schöffl F
,
Key JL
.
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Soybeans, Glycine max, synthesize a family of low-molecular-weight heat shock (HS) proteins in response to HS. The DNA sequences of two genes encoding 17.5- and 17.6-kilodalton HS proteins were determined. Nuclease S1 mapping of the corresponding mRNA indicated multiple start termini at the 5' end and multiple stop termini at the 3' end. These two genes were compared with two other soybean HS genes of similar size. A comparison among the 5' flanking regions encompassing the presumptive HS promoter of the soybean HS-protein genes demonstrated this region to be extremely homologous. Analysis of the DNA sequences in the 5' flanking regions of the soybean genes with the corresponding regions of Drosophila melanogaster HS-protein genes revealed striking similarity between plants and animals in the presumptive promoter structure of thermoinducible genes. Sequences related to the Drosophila HS consensus regulatory element were found 57 to 62 base pairs 5' to the start of transcription in addition to secondary HS consensus elements located further upstream. Comparative analysis of the deduced amino acid sequences of four soybean HS proteins illustrated that these proteins were greater than 90% homologous. Comparison of the amino acid sequence for soybean HS proteins with other organisms showed much lower homology (less than 20%). Hydropathy profiles for Drosophila, Xenopus, Caenorhabditis elegans, and G. max HS proteins showed a similarity of major hydrophilic and hydrophobic regions, which suggests conservation of functional domains for these proteins among widely dispersed organisms.
Arrigo,
Immunofluorescence localization of a small heat shock protein (hsp 23) in salivary gland cells of Drosophila melanogaster.
1981, Pubmed
Arrigo,
Immunofluorescence localization of a small heat shock protein (hsp 23) in salivary gland cells of Drosophila melanogaster.
1981,
Pubmed
Arrigo,
Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells.
1980,
Pubmed
Ashburner,
The induction of gene activity in drosophilia by heat shock.
1979,
Pubmed
Ayme,
Nucleotide sequences responsible for the thermal inducibility of the Drosophila small heat-shock protein genes in monkey COS cells.
1985,
Pubmed
Benoist,
The ovalbumin gene-sequence of putative control regions.
1980,
Pubmed
Bienz,
Expression of a Drosophila heat-shock protein in Xenopus oocytes: conserved and divergent regulatory signals.
1982,
Pubmed
,
Xenbase
Bienz,
Developmental control of the heat shock response in Xenopus.
1984,
Pubmed
,
Xenbase
Blattner,
Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
1978,
Pubmed
Corces,
Integration, transcription, and control of a Drosophila heat shock gene in mouse cells.
1981,
Pubmed
Czarnecka,
DNA sequence and transcript mapping of a soybean gene encoding a small heat shock protein.
1985,
Pubmed
DiDomenico,
The heat shock response is self-regulated at both the transcriptional and posttranscriptional levels.
1982,
Pubmed
DiDomenico,
Heat shock and recovery are mediated by different translational mechanisms.
1982,
Pubmed
Dudler,
Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.
1984,
Pubmed
,
Xenbase
Favaloro,
Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
1980,
Pubmed
Ingolia,
Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin.
1982,
Pubmed
Karin,
Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
,
Pubmed
Karn,
Novel bacteriophage lambda cloning vector.
1980,
Pubmed
Kelley,
Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species.
1982,
Pubmed
Key,
Heat shock proteins of higher plants.
1981,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lin,
Acquisition of Thermotolerance in Soybean Seedlings : Synthesis and Accumulation of Heat Shock Proteins and their Cellular Localization.
1984,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McMahon,
Inducible expression of a cloned heat shock fusion gene in sea urchin embryos.
1984,
Pubmed
Mirault,
Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells.
1982,
Pubmed
Nagao,
Multigene family of actin-related sequences isolated from a soybean genomic library.
1981,
Pubmed
Nordheim,
Negatively supercoiled simian virus 40 DNA contains Z-DNA segments within transcriptional enhancer sequences.
,
Pubmed
O'Connor,
Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation.
1981,
Pubmed
Parker,
A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
1984,
Pubmed
Pelham,
A synthetic heat-shock promoter element confers heat-inducibility on the herpes simplex virus thymidine kinase gene.
1982,
Pubmed
,
Xenbase
Pelham,
A regulatory upstream promoter element in the Drosophila hsp 70 heat-shock gene.
1982,
Pubmed
Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed
Pustell,
A convenient and adaptable package of DNA sequence analysis programs for microcomputers.
1982,
Pubmed
Russnak,
Cloning and analysis of cDNA sequences coding for two 16 kilodalton heat shock proteins (hsps) in Caenorhabditis elegans: homology with the small hsps of Drosophila.
1983,
Pubmed
Schöffl,
An analysis of mRNAs for a group of heat shock proteins of soybean using cloned cDNAs.
1982,
Pubmed
Schöffl,
The DNA sequence analysis of soybean heat-shock genes and identification of possible regulatory promoter elements.
1984,
Pubmed
Slightom,
Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
1980,
Pubmed
Southgate,
Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B.
1983,
Pubmed
Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed
Vierling,
Ribulose 1,5-Bisphosphate Carboxylase Synthesis during Heat Shock.
1985,
Pubmed
Wu,
Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
,
Pubmed
Wu,
Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin.
,
Pubmed