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Chromosoma
1986 Jan 01;943:217-27. doi: 10.1007/bf00288496.
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Changes in DNA topology during spermatogenesis.
Risley MS
,
Einheber S
,
Bumcrot DA
.
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DNA topology in histone- and protamine-depleted nuclei (nucleoids) from somatic cells, sperm, and spermatogenic cells was studied to determine if the superhelical configuration of DNA looped domains is altered during spermatogenesis. The expansion and contraction of nucleoid DNA was measured with a fluorescence microscope following exposure of nucleoids to different concentrations of ethidium bromide (EB). Nucleoids from Xenopus laevis erythrocytes, primary spermatocytes, and round spermatids, and from Rana catesbeiana sperm all exhibited a biphasic change (condensed-relaxed-condensed) in size as a function of exposure to increasing concentrations (0.5-100 micrograms/ml) of EB, indicating that they contain negatively supercoiled DNA. In contrast, DNA in sperm nucleoids from Xenopus laevis and Bufo fowleri was relaxed and expanded at low (0.5-6 micrograms/ml) EB concentrations, but became gradually condensed as the EB concentration was increased (6-100 micrograms/ml). Nucleoids prepared from all cell types retained the general shape of the nucleus regardless of the superhelical configuration of the nucleoid DNA. Sperm nucleoid DNA condensed by 100 micrograms/ml EB was relaxed by exposure to UV light, DNase I, proteinase K, or 4 M urea, but not by RNase A or 10 mM dithiothreitol. These results demonstrate that the DNA in sperm nucleoids is constrained in domains of supercoiling by nonbasic nuclear proteins. Negatively supercoiled DNA is present in nucleoids from cells with a full complement of histones, including Rana sperm, but not in nucleoids from Xenopus and Bufo sperm in which histones are replaced by "intermediate-type" protamines. Histone replacement in these species, therefore, is accompanied by unfolding of nucleosomal DNA and active removal of the negative supercoils. Results presented also suggest an important role for the nonbasic nuclear proteins of sperm in the morphogenesis of the nucleus and the arrangement of DNA.
Adolph,
Organization of chromosomes in HeLa cells: isolation of histone-depleted nuclei and nuclear scaffolds.
1980, Pubmed
Adolph,
Organization of chromosomes in HeLa cells: isolation of histone-depleted nuclei and nuclear scaffolds.
1980,
Pubmed
Adolph,
Role of nonhistone proteins in metaphase chromosome structure.
1977,
Pubmed
Alder,
Electrophoretic analysis of liver and testis histones of the frog Rana pipiens.
1975,
Pubmed
Ausió,
Nuclear proteins and the organization of chromatin in spermatozoa of Mytilus edulis.
1982,
Pubmed
Balhorn,
A model for the structure of chromatin in mammalian sperm.
1982,
Pubmed
Benavente,
Change of karyoskeleton during spermatogenesis of Xenopus: expression of lamin LIV, a nuclear lamina protein specific for the male germ line.
1985,
Pubmed
,
Xenbase
Benyajati,
Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.
1976,
Pubmed
Bode,
On the competition between protamines and histones: studies directed towards the understanding of spermiogenesis.
1977,
Pubmed
Buongiorno-Nardelli,
A relationship between replicon size and supercoiled loop domains in the eukaryotic genome.
1982,
Pubmed
,
Xenbase
Cook,
Characterization of nuclear structures containing superhelical DNA.
1976,
Pubmed
Cook,
Conformational constraints in nuclear DNA.
1976,
Pubmed
Cornudella,
Nucleosome organization during germ cell development in the sea cucumber Holothuria tubulosa.
1979,
Pubmed
Easton,
High-resolution electrophoretic analysis of the histones from embryos and sperm of Arbacia punctulata.
1972,
Pubmed
Gusse,
Molecular structure of chromatin during sperm differentiation of the dogfish Scyliorhinus caniculus (L.).
1980,
Pubmed
Gusse,
Electron microscope evidence for the presence of globular structures in different sperm chromatins.
1980,
Pubmed
Honda,
The subunit structure of chromatin: characteristics of nucleohistone and nucleoprotamine from developing trout testis.
1974,
Pubmed
Igó-Kemenes,
Domains in chromatin structure.
1978,
Pubmed
Kasinsky,
On the diversity of sperm histones in the vertebrates: IV. Cytochemical and amino acid analysis in Anura.
1985,
Pubmed
,
Xenbase
Kaufmann,
A subset of non-histone nuclear proteins reversibly stabilized by the sulfhydryl cross-linking reagent tetrathionate. Polypeptides of the internal nuclear matrix.
1984,
Pubmed
Kierszenbaum,
The packaging unit: a basic structural feature for the condensation of late cricket spermatid nuclei.
1978,
Pubmed
Kierszenbaum,
Structural and transcriptional features of the mouse spermatid genome.
1975,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lebkowski,
Evidence for two levels of DNA folding in histone-depleted HeLa interphase nuclei.
1982,
Pubmed
Lebkowski,
Non-histone proteins and long-range organization of HeLa interphase DNA.
1982,
Pubmed
Lewis,
Higher order metaphase chromosome structure: evidence for metalloprotein interactions.
1982,
Pubmed
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
Loir,
Interactions of nuclear proteins with DNA, during sperm differentiation in the ram.
1985,
Pubmed
Marsden,
Metaphase chromosome structure: evidence for a radial loop model.
1979,
Pubmed
Marushige,
Complete displacement of somatic histones during transformation of spermatid chromatin: a model experiment.
1976,
Pubmed
McMaster-Kaye,
Organization of chromatin during spermiogenesis: beaded fibers, partly beaded fibers, and loss of nucleosomal structure.
1980,
Pubmed
Miller,
Morphological studies of transcription.
1972,
Pubmed
Mirkovitch,
Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.
1984,
Pubmed
Moses,
Positive contrast staining and protected drying of surface spreads: electron microscopy of the synaptonemal complex by a new method.
1976,
Pubmed
Muñoz-Guerra,
Structural organization of sperm chromatin from the fish Carassius auratus.
1982,
Pubmed
Oakley,
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
1980,
Pubmed
Palau,
Histones from sperm of the sea urchin Arbacia lixula.
1969,
Pubmed
Paulson,
The structure of histone-depleted metaphase chromosomes.
1977,
Pubmed
Phelan,
An unusual group of lysine-rich histones from gonads of a sea cucumber, Holothuria tubulosa.
1972,
Pubmed
Pruslin,
Proteins of de-membraned protamine-depleted mouse sperm. Homology with proteins of somatic cell nuclear envelope/matrix.
1983,
Pubmed
Risley,
H1 histone variants in Xenopus laevis.
1981,
Pubmed
,
Xenbase
Simon,
An octamer of histones H3 and H4 forms a compact complex with DNA of nucleosome size.
1978,
Pubmed
Sobhon,
Electron microscopic and biochemical analyses of the organization of human sperm chromatin decondensed with sarkosyl and dithiothreitol.
1982,
Pubmed
Spadafora,
The DNA repeat lengths in chromatins from sea urchin sperm and gastrule cells are markedly different.
1976,
Pubmed
Stick,
The disappearance of the nuclear lamina during spermatogenesis: an electron microscopic and immunofluorescence study.
1982,
Pubmed
Vogelstein,
Supercoiled loops and eucaryotic DNA replicaton.
1980,
Pubmed
Wagner,
Human sperm chromatin has a nucleosomal structure.
1981,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase