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Transcription of class III genes in cell-free extracts from the nematode Caenorhabditis elegans.
Honda BM
,
Devlin RH
,
Nelson DW
,
Khosla M
.
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Using the nematode Caenorhabditis elegans as a model organism, we have prepared cell-free extracts which accurately transcribe cloned homologous 5S RNA genes in vitro. These extracts also transcribe cloned tRNA genes, and actively process the resulting products. Unlike tRNA genes, transcription of 5S DNA shows some species specificity: C. elegans extracts do not transcribe Xenopus 5S RNA genes, nor does a Xenopus extract efficiently transcribe the heterologous nematode 5S DNA. However, addition of limiting amounts of C. elegans fractions permits Xenopus extracts to transcribe nematode 5S RNA genes. This apparent biochemical "complementation" may provide an assay to purify 5S RNA gene-specific factors from C. elegans.
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985, Pubmed,
Xenbase
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985,
Pubmed
,
Xenbase
Brenner,
The genetics of Caenorhabditis elegans.
1974,
Pubmed
Brown,
The role of stable complexes that repress and activate eucaryotic genes.
1984,
Pubmed
,
Xenbase
Ciliberto,
Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
1982,
Pubmed
,
Xenbase
Dudler,
Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.
1984,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase
Laskey,
Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
1977,
Pubmed
Marx,
Caenorhabditis elegans: getting to know you.
1984,
Pubmed
Mishima,
Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.
1982,
Pubmed
Rose,
The linkage mapping of cloned restriction fragment length differences in Caenorabditis elegans.
1982,
Pubmed
Sanford,
RNA polymerase II from wild type and alpha-amanitin-resistant strains of Caenorhabditis elegans.
1983,
Pubmed
Schaack,
The extent of a eukaryotic tRNA gene. 5'- and 3'-flanking sequence dependence for transcription and stable complex formation.
1984,
Pubmed
Sprague,
5' flanking sequence signals are required for activity of silkworm alanine tRNA genes in homologous in vitro transcription systems.
1980,
Pubmed
,
Xenbase
Sulston,
The DNA of Caenorhabditis elegans.
1974,
Pubmed
Sulston,
The embryonic cell lineage of the nematode Caenorhabditis elegans.
1983,
Pubmed
Swanson,
Critical periods in the development of the Caenorhabditis elegans dauer larva.
1981,
Pubmed
Tschudi,
Sequence and heterogeneity in the 5S RNA gene cluster of Drosophila melanogaster.
1980,
Pubmed
Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed