J Neurosci Methods 1986 Aug 01;172-3:109-19. doi: 10.1016/0165-0270(86)90064-6.

A radiolabelled pulse for the simultaneous study of anterograde and retrograde axonal transport.

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A technique is described for producing a pulse of [35S]methionine-labelled material which is axonally transported in amphibian sciatic nerve maintained in vitro. Using a position-sensitive detector of ionizing radiation, it is possible to continuously observe the movement of the pulse in the anterograde direction and, following turnaround at a ligature, the movement of a fraction of the pulse in the retrograde direction. Two sources of contaminant activity, which would otherwise interfere with observation of the pulse, are discussed and shown to be avoidable.

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