Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Expression of simian virus 40 early and late genes in mouse oocytes and embryos.
Chalifour LE
,
Wirak DO
,
Wassarman PM
,
DePamphilis ML
.
???displayArticle.abstract???
Simian virus 40 (SV40) large- and small-tumor antigens (T-Ag, t-Ag) are normally synthesized early after infection of either permissive (monkey) or nonpermissive (mouse) fibroblasts, whereas an equivalent amount of viral coat protein (V-Ag) is observed late after infection of permissive cells and only after viral DNA replication has occurred. To determine whether or not expression of these genes is regulated in the same manner during early mammalian development, SV40 DNA was injected into the nuclei of mouse oocytes and one- and two-cell embryos. In oocytes, about three times more V-Ag was produced than T-Ag, and both were synthesized concomitantly in the same cells. Viral mRNA and proteins synthesized in oocytes comigrated during gel electrophoresis with the same products synthesized in SV40-infected monkey cells. Viral gene expression required circular DNA molecules injected into the nuclei of transcriptionally and translationally active cells. Injected DNA was stable and underwent conformational changes consistent with chromatin assembly. Oocytes did not replicate either polyomavirus or SV40 DNA. Thus, the temporal order of viral gene expression is circumvented in mouse germ cells, allowing these proteins to be expressed concurrently and in equivalent amounts with no requirement for DNA replication. However, in preimplantation embryos, neither T-Ag nor V-Ag was detected by immunoprecipitation although T-Ag synthesis was demonstrated as a specific requirement for SV40 DNA replication. Thus, viral gene expression in mouse embryos as early as the one-cell stage was reduced at least 500-fold relative to that in oocytes. Similarities between SV40 gene expression in mouse oocytes and that in Xenopus oocytes suggest that germ cells in higher animals share common regulatory mechanisms.
Abramczuk,
Infection of mouse preimplantation embryos with simian virus 40 and polyoma virus.
1978, Pubmed
Abramczuk,
Infection of mouse preimplantation embryos with simian virus 40 and polyoma virus.
1978,
Pubmed
Bendig,
Fidelity of transcription of Xenopus laevis globin genes injected into Xenopus laevis oocytes and unfertilized eggs.
1984,
Pubmed
,
Xenbase
Birkenmeier,
Characterization of simian virus 40 tsA58 transcriptional intermediates at restrictive temperatures: relationship between DNA replication and transcription.
1977,
Pubmed
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Brady,
trans Activation of the simian virus 40 late transcription unit by T-antigen.
1985,
Pubmed
Buchman,
Complex regulation of simian virus 40 early-region transcription from different overlapping promoters.
1984,
Pubmed
Chia,
Fate of viral DNA in nonpermissive cells infected with simian virus 40.
1981,
Pubmed
Contreras,
Evidence for the direct involvement of DNA replication origin in synthesis of late SV40 RNA.
1982,
Pubmed
,
Xenbase
Ernoult-Lange,
Evidence of transcription from the late region of the integrated simian virus 40 genome in transformed cells: location of the 5' ends of late transcripts in cells abortively infected and in cells transformed by simian virus 40.
1983,
Pubmed
Etkin,
Analysis of the mechanisms involved in gene regulation and cell differentiation by microinjection of purified genes and somatic cell nuclei into amphibian oocytes and eggs.
1982,
Pubmed
,
Xenbase
Gargiulo,
Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
1984,
Pubmed
,
Xenbase
Gottesman,
Purification and characterization of a transformation-dependent protein secreted by cultured murine fibroblasts.
1981,
Pubmed
Graessmann,
Regulatory function of simian virus 40 DNA replication for late viral gene expression.
1977,
Pubmed
Graessmann,
Regulatory mechanism of simian virus 40 gene expression in permissive and in nonpermissive cells.
1976,
Pubmed
Gurney,
Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens.
1980,
Pubmed
Harland,
Regulated replication of DNA microinjected into eggs of Xenopus laevis.
1980,
Pubmed
,
Xenbase
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Hartzell,
The simian virus 40 minimal origin and the 72-base-pair repeat are required simultaneously for efficient induction of late gene expression with large tumor antigen.
1984,
Pubmed
Huang,
Structure and arrangement of the beta-tubulin genes of Leishmania tropica.
1984,
Pubmed
Keller,
Analysis of an activatable promoter: sequences in the simian virus 40 late promoter required for T-antigen-mediated trans activation.
1985,
Pubmed
Kelly,
Tumor viruses and early mouse embryos.
1982,
Pubmed
Khoury,
Regulation of early and late simian virus 40 transcription: overproduction of early viral RNA in the absence of a functional T-antigen.
1977,
Pubmed
Kopchick,
Differences in intracellular DNA ligation after microinjection and transfection.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Lebowitz,
Organization and transcription of the simian virus 40 genome.
1979,
Pubmed
Lusky,
Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
1981,
Pubmed
Mertz,
Linear DNA does not form chromatin containing regularly spaced nucleosomes.
1982,
Pubmed
,
Xenbase
Michaeli,
Regulation of simian virus 40 gene expression in Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Miller,
Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA.
1982,
Pubmed
,
Xenbase
Miller,
Template structural requirements for transcription in vivo by RNA polymerase II.
1982,
Pubmed
,
Xenbase
Parker,
Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.
1979,
Pubmed
Stern,
Meiotic maturation of the mammalian oocyte in vitro: effect of dibutyryl cyclic AMP on protein synthesis.
1974,
Pubmed
Tabor,
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
1985,
Pubmed
Wassarman,
Oocyte development in the mouse: an ultrastructural comparison of oocytes isolated at various stages of growth and meiotic competence.
1978,
Pubmed
Wickens,
Post-transcriptional processing of simian virus 40 late transcripts in injected frog oocytes.
1983,
Pubmed
,
Xenbase
Wirak,
Sequence-dependent DNA replication in preimplantation mouse embryos.
1985,
Pubmed
,
Xenbase
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase