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Signals provided in vivo by human rIL-2 and Con A can switch hapten-specific tolerance from unresponsiveness to responsiveness in the South African clawed toad.
Ruben LN
,
Clothier RH
,
Mirchandani M
,
Wood P
,
Balls M
.
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Injection in vivo of trinitrobenzene sulphonic acid (TNBS) will conjugate trinitrophenyl (TNP) to the cells and proteins of rodents, and induce hapten-specific tolerance to this epitope. However, the induction of hapten-specific tolerance by this method in the South African clawed toad, Xenopus laevis, is restricted to its subsequent presentation on Ficoll or polyvinylpyrrolidone (PVP). This restricted tolerance depends on the stimulation of an N-methyl-N-nitrosourea (NMU)-insensitive, cyclophosphamide (CyP)-sensitive hapten-specific (suppressor) population that is functionally demonstrable in vitro. Unresponsiveness to TNP-Ficoll can be switched to responsiveness by injection in vivo of recombinant DNA-produced human IL-2 (rIL-2) or the plant-derived lectin, concanavalin A (Con A). Responsiveness to TNP-Ficoll requires thymic presence, although thymic extracts from rIL-2- or Con A-injected toads will suffice. Unresponsiveness to TNP-PVP can also be broken by these reagents, but thymic presence is not required with this immunogen. TNP-Ficoll responses are thymus requiring, while those to TNP-PVP are not, in the toad. Since TNBS failed to stimulate hapten-specific tolerance to a secondary challenge to TNP-Ficoll, we suggest that the suppressor function involved in the establishment of the unresponsive state may act on the differentiation, rather than on the function, of the relevant B cells.
Chiller,
Kinetic differences in unresponsiveness of thymus and bone marrow cells.
1971, Pubmed
Chiller,
Kinetic differences in unresponsiveness of thymus and bone marrow cells.
1971,
Pubmed
Colizzi,
In vivo activity of interleukin-2: conversion of a stimulus causing unresponsiveness to a stimulus causing contact hypersensitivity by the injection of interleukin-2.
1985,
Pubmed
Edwards,
Surface characteristics of spleen cell-erythrocyte rosette formation in the grass frog Rana pipiens.
1975,
Pubmed
Fidler,
Immunological tolerance to a hapten. I. Induction and maintenance of tolerance to trinitrophenyl with trinitrobenzene sulfonic acid.
1973,
Pubmed
Horton,
Use of different carriers to demonstrate thymic-dependent and thymic-independent anti-trinitrophenyl reactivity in the ambhibian Xenopus laevis.
1979,
Pubmed
,
Xenbase
Köhler,
Clonal depletion in neonatal tolerance.
1974,
Pubmed
Lake,
Regulation of the immune response to polyvinylpyrrolidone:effect of antilymphocyte serum on the response of normal and nude mice.
1976,
Pubmed
Lattime,
L3T4+, B2A2+ thymocytes from infant mice produce IL 2 after interaction with accessory cells expressing self class II antigens.
1986,
Pubmed
Louis,
Fate of antigen-binding cells in unresponsive and immune mice.
1973,
Pubmed
Mette,
Trinitrobenzene sulfonic acid effects in two amphibian model systems.
1980,
Pubmed
,
Xenbase
Meucci,
Membrane defects of the tolerant B cell. IV. Failure to accelerate antigen receptor loss.
1986,
Pubmed
Mosier,
The ontogeny of thymic independent antibody responses in vitro in normal mice and mice with an X-linked B cell defect.
1977,
Pubmed
Piantelli,
Proliferation of phenotypically immature human thymocytes with and without interleukin 2 receptors.
1986,
Pubmed
Rittenberg,
Immunogenicity of trinitrophenyl-hemocyanin: production of primary and secondary anti-hapten precipitins.
1966,
Pubmed
Ruben,
Thymic involvement in memory responses after primary challenge with TNP-Ficoll in Xenopus laevis, the South African clawed toad.
1986,
Pubmed
,
Xenbase
Ruben,
Phenotypic restriction of antigen-binding specificity on immunized amphibian spleen cells.
1977,
Pubmed
,
Xenbase
Ruben,
In vitro thymus suppression of hemagglutinin production in Xenopus laevis: location, drug and temperature sensitivity.
1984,
Pubmed
,
Xenbase
Ruben,
Murine and human interleukin 2 can substitute for the thymus in immune responses to TNP-Ficoll in Xenopus laevis, the South African clawed toad.
1985,
Pubmed
,
Xenbase
Sieckmann,
An in vitro model for induction of immunologic unresponsiveness to turkey gamma-globulin in primed mouse spleen cells, II. Antigen-specific suppressor cells.
1979,
Pubmed
Tochinai,
Demonstration of thymus-independent immune system in Xenopus laevis. Response to polyvinylpyrrolidone.
1976,
Pubmed
,
Xenbase
Turk,
Selective depletion of lymphoid tissue by cyclophosphamide.
1972,
Pubmed
Watkins,
The phylogeny of interleukin-2.
1985,
Pubmed
,
Xenbase
