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An alternative protein factor which binds the internal promoter of Xenopus 5S ribosomal RNA genes.
Barrett P
,
Sommerville J
.
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In small oocytes of Xenopus species, two sets of 5S RNA genes, oocyte-type and somatic-type, are fully activated. The 5S RNA transcripts are temporarily stored, half in association with TFIIIA to form a 7S particle, the other half in association with tRNA and two proteins (p48 and p43) to form a 42S particle. It has been established previously that TFIIIA binds to the internal control region of 5S RNA genes and promotes their transcription. Here we show that protein can be translocated from the 42S particles to 5S RNA genes, but only after treatment of the particles with ribonuclease. Nevertheless, once transferred, stable protein-DNA complexes are formed and DNase-protection experiments show that binding is specific to the gene promoter, covering exactly the same sequence as TFIIIA. The DNA-binding protein is identified as p48 which, after isolation by ion-exchange chromatography, will bind to 5S RNA genes in the absence of ribonuclease.
Barrett,
Specific interaction of proteins with 5 S RNA and tRNA in the 42 S storage particle of Xenopus oocytes.
1983, Pubmed,
Xenbase
Barrett,
Specific interaction of proteins with 5 S RNA and tRNA in the 42 S storage particle of Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Barrett,
Immunological identity of proteins that bind stored 5S RNA in Xenopus oocytes.
1984,
Pubmed
,
Xenbase
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase
Ciliberto,
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
1983,
Pubmed
,
Xenbase
Denis,
[Biochemical studies on oogenesis. 1. Intracellular RNA distribution in small oocytes of Xenopus laevis].
1972,
Pubmed
,
Xenbase
Denis,
Biochemical research on oogenesis. Oocytes and liver cells of the teleost fish Tinca tinca contain different kinds of 5S RNA.
1977,
Pubmed
Denis,
Thesaurisomes, a novel kind of nucleoprotein particle.
1983,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Ford,
Non-coordinated accumulation and synthesis of 5S ribonucleic acid by ovaries of Xenopus laevis.
1971,
Pubmed
,
Xenbase
Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Gilbert,
Temporal order of replication of Xenopus laevis 5S ribosomal RNA genes in somatic cells.
1986,
Pubmed
,
Xenbase
Harrison,
Gene regulation. Fingers and DNA half-turns.
,
Pubmed
Johnson,
Distribution and utilization of 5 S-RNA-binding proteins during the development of Xenopus oocytes.
1984,
Pubmed
,
Xenbase
Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Müller,
Sequence of a 3.18 kb tandem repeat of Xenopus laevis DNA containing 8 tRNA genes.
1987,
Pubmed
,
Xenbase
Pelham,
Related 5S RNA transcription factors in Xenopus oocytes and somatic cells.
1981,
Pubmed
,
Xenbase
Picard,
Isolation of a 7S particle from Xenopus laevis oocytes: a 5S RNA-protein complex.
1979,
Pubmed
,
Xenbase
Picard,
Biochemical Research on oogenesis. Composition of the 42-S storage particles of Xenopus laevix oocytes.
1980,
Pubmed
,
Xenbase
Reynolds,
Control of 5S RNA transcription in Xenopus somatic cell chromatin: activation with an oocyte extract.
1983,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Shastry,
Altered levels of a 5 S gene-specific transcription factor (TFIIIA) during oogenesis and embryonic development of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase
Wormington,
A quantitative assay for Xenopus 5S RNA gene transcription in vitro.
1981,
Pubmed
,
Xenbase
