Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Muscle Res Cell Motil
2004 Jan 01;254-5:285-96. doi: 10.1007/s10974-004-8716-8.
Show Gene links
Show Anatomy links
Effects of strain on contractile force and number of sarcomeres in series of Xenopus laevis single muscle fibres during long-term culture.
Jaspers RT
,
Feenstra HM
,
Verheyen AK
,
van der Laarse WJ
,
Huijing PA
.
???displayArticle.abstract???
The aim of the present study is to test whether mechanical strain uniquely regulates muscle fibre atrophy/hypertrophy and adaptation of the number of sarcomeres in series within mature muscle fibres in vitro . Mature single muscle fibres from Xenopus laevis illiofibularis muscle were cultured (4-97 days) while kept at negative strain ( approximately 20% below passive slack length, 'short fibres') or at positive strain ( approximately 5% over passive slack length, 'long fibres'). Before and after culture the number of sarcomeres in series was determined using laser diffraction. During culture, twitch and tetanic force characteristics were measured every day. Survival time of long fibres was substantially less than that of short fibres. Of the long fibres 40% died or became inexcitable within 1 week, whereas this did not occur for short fibres. During culture, twitch and tetanic force of all short fibres increased substantially. Regression analysis showed that the post-culture number of sarcomeres in series was not significantly changed compared to the number before culture. It is concluded that culture at negative strain does not result in atrophy or a reduction of the number of sarcomeres in series, even after 97 days. For the long fibres we did not detect any hypertrophy as tetanic force remained stable or decreased slowly, while twitch force varied. Regression analysis of the change of the number of sarcomeres in series as a function of the culture time showed a positive slope ( P=0.054). Two out of four long fibres that were cultured for at least 2 weeks showed an increase in the number of sarcomeres of 4-5%. Compared with in vivo adaptation to mechanical stimuli this is much less than would be expected. The data suggest that strain may not be the only factor that regulates hypertrophy and the number of sarcomeres in series.
Adams,
Localized infusion of IGF-I results in skeletal muscle hypertrophy in rats.
1998, Pubmed
Adams,
Localized infusion of IGF-I results in skeletal muscle hypertrophy in rats.
1998,
Pubmed
Ahn,
In vivo and in vitro heterogeneity of segment length changes in the semimembranosus muscle of the toad.
2003,
Pubmed
Barton-Davis,
Contribution of satellite cells to IGF-I induced hypertrophy of skeletal muscle.
1999,
Pubmed
Bekoff,
Properties of isolated adult rat muscle fibres maintained in tissue culture.
1977,
Pubmed
Belcastro,
Exercise-induced muscle injury: a calpain hypothesis.
1998,
Pubmed
Berchtold,
Calcium ion in skeletal muscle: its crucial role for muscle function, plasticity, and disease.
2000,
Pubmed
Bland,
Calculating correlation coefficients with repeated observations: Part 1--Correlation within subjects.
1995,
Pubmed
Borgers,
Localization of calcium in skeletal and cardiac muscle.
1984,
Pubmed
Burkholder,
Sarcomere number adaptation after retinaculum transection in adult mice.
1998,
Pubmed
Carson,
Stretch overload-induced satellite cell activation in slow tonic muscle from adult and aged Japanese quail.
1996,
Pubmed
Casaburi,
Skeletal muscle function in COPD.
2000,
Pubmed
Clark,
Mechanisms of exercise intolerance in cardiac failure: abnormalities of skeletal muscle and pulmonary function.
1994,
Pubmed
Crawford,
The growth of striated muscle immobilized in extension.
1973,
Pubmed
Feng,
The effect of length on the resting metabolism of muscle.
1932,
Pubmed
Glavinović,
Morphological and physiological changes in dissociated adult frog muscle fibres after prolonged culturing.
1983,
Pubmed
,
Xenbase
Goldspink,
Changes in striated muscle fibres during contraction and growth with particular reference to myofibril splitting.
1971,
Pubmed
Goldspink,
Effect of denervation on the adaptation of sarcomere number and muscle extensibility to the functional length of the muscle.
1974,
Pubmed
Goldspink,
Changes in muscle mass and phenotype and the expression of autocrine and systemic growth factors by muscle in response to stretch and overload.
1999,
Pubmed
Gordon,
The variation in isometric tension with sarcomere length in vertebrate muscle fibres.
1966,
Pubmed
Gulve,
Regulation of protein synthesis and degradation in L8 myotubes. Effects of serum, insulin and insulin-like growth factors.
1989,
Pubmed
Hawke,
Myogenic satellite cells: physiology to molecular biology.
2001,
Pubmed
Hayat,
Effects of denervation on the reduction of sarcomere number in cat soleus muscle immobilized in shortened position during seven days.
1978,
Pubmed
Heslinga,
Growth and immobilization effects on sarcomeres: a comparison between gastrocnemius and soleus muscles of the adult rat.
1995,
Pubmed
Heslinga,
Muscle length-force characteristics in relation to muscle architecture: a bilateral study of gastrocnemius medialis muscles of unilaterally immobilized rats.
1993,
Pubmed
Huijing,
Extramuscular myofascial force transmission within the rat anterior tibial compartment: proximo-distal differences in muscle force.
2001,
Pubmed
Huijing,
Myofascial force transmission: muscle relative position and length determine agonist and synergist muscle force.
2003,
Pubmed
Jaspers,
Twitch and tetanic tension during culture of mature Xenopus laevis single muscle fibres.
2001,
Pubmed
,
Xenbase
Koh,
Excursion is important in regulating sarcomere number in the growing rabbit tibialis anterior.
1998,
Pubmed
Lee-De Groot,
Twitch characteristics and energy metabolites of mature muscle fibres of Xenopus laevis in culture.
1996,
Pubmed
,
Xenbase
Maas,
Intermuscular interaction via myofascial force transmission: effects of tibialis anterior and extensor hallucis longus length on force transmission from rat extensor digitorum longus muscle.
2001,
Pubmed
McCall,
Maintenance of myonuclear domain size in rat soleus after overload and growth hormone/IGF-I treatment.
1998,
Pubmed
McKoy,
Expression of insulin growth factor-1 splice variants and structural genes in rabbit skeletal muscle induced by stretch and stimulation.
1999,
Pubmed
Mitchell,
Calcineurin differentially regulates maintenance and growth of phenotypically distinct muscles.
2002,
Pubmed
Monier,
Insulin affects only initiation and not elongation in protein synthesis in soleus muscles of lean and obese mice.
1982,
Pubmed
Morgan,
New insights into the behavior of muscle during active lengthening.
1990,
Pubmed
Mozdziak,
Hoechst fluorescence intensity can be used to separate viable bromodeoxyuridine-labeled cells from viable non-bromodeoxyuridine-labeled cells.
2000,
Pubmed
Muscatello,
On the differential response of sarcoplasm and myoplasm to denervation in frog muscle.
1965,
Pubmed
Ohira,
Organ culture of frog muscle: maintenance of mass, enzyme levels, and contractile force.
1989,
Pubmed
Oishi,
Clenbuterol induces expression of multiple myosin heavy chain isoforms in rat soleus fibres.
2002,
Pubmed
PAGE,
FILAMENT LENGTHS IN STRIATED MUSCLE.
1963,
Pubmed
Pappas,
Nonuniform shortening in the biceps brachii during elbow flexion.
2002,
Pubmed
Perrone,
Collagen and stretch modulate autocrine secretion of insulin-like growth factor-1 and insulin-like growth factor binding proteins from differentiated skeletal muscle cells.
1995,
Pubmed
Phelan,
Effect of radiation on satellite cell activity and protein expression in overloaded mammalian skeletal muscle.
1997,
Pubmed
Rosenblatt,
Satellite cell activity is required for hypertrophy of overloaded adult rat muscle.
1994,
Pubmed
Sayegh,
In vivo rates of protein synthesis in brain, muscle, and liver of five vertebrate species.
1989,
Pubmed
Shevchenko,
Identification, characterization and partial purification of a thiol-protease which cleaves specifically the skeletal muscle ryanodine receptor/Ca2+ release channel.
1998,
Pubmed
Shimizu,
Insulin and insulinlike growth factor receptors and responses in cultured human muscle cells.
1986,
Pubmed
Smith,
Varieties of fast and slow extrafusal muscle fibres in amphibian hind limb muscles.
1973,
Pubmed
,
Xenbase
Snowdowne,
The effect of stretch on sarcoplasmic free calcium of frog skeletal muscle at rest.
1986,
Pubmed
Spector,
Architectural alterations of rat hind-limb skeletal muscles immobilized at different lengths.
1982,
Pubmed
Sugden,
Regulation of protein turnover in skeletal and cardiac muscle.
1991,
Pubmed
Swatland,
Analysis of growth in a complex muscle (M. supracoracoideus, Anas platyrhynchos).
1980,
Pubmed
Tabary,
Physiological and structural changes in the cat's soleus muscle due to immobilization at different lengths by plaster casts.
1972,
Pubmed
Tabary,
Experimental rapid sarcomere loss with concomitant hypoextensibility.
1981,
Pubmed
Tardieu,
[Change in the number of sarcomeres and in isometric tetanic tension after immobilization of the cat anterior tibialis muscle at different lengths].
1974,
Pubmed
Tardieu,
[Adjustment of the number of sarcomeres of the muscle fiber to its required length. Consequence of this regulation and of its disorders observed in neurology].
1973,
Pubmed
V Euler,
Some factors influencing the heat production of muscle after stretching.
1935,
Pubmed
Vandenburgh,
Insulin and IGF-I induce pronounced hypertrophy of skeletal myofibers in tissue culture.
1991,
Pubmed
Wang,
Single molecule measurements of titin elasticity.
2001,
Pubmed
Willems,
Heterogeneity of mean sarcomere length in different fibres: effects on length range of active force production in rat muscle.
1994,
Pubmed
Williams,
Changes in sarcomere length and physiological properties in immobilized muscle.
1978,
Pubmed
Williams,
Longitudinal growth of striated muscle fibres.
1971,
Pubmed
Winchester,
Satellite cell activation in the stretch-enlarged anterior latissimus dorsi muscle of the adult quail.
1991,
Pubmed
Yang,
Changes in muscle fibre type, muscle mass and IGF-I gene expression in rabbit skeletal muscle subjected to stretch.
1997,
Pubmed
Yang,
Cloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch.
1996,
Pubmed
Yang,
Different roles of the IGF-I Ec peptide (MGF) and mature IGF-I in myoblast proliferation and differentiation.
2002,
Pubmed
Yokochi,
Motor function of infants with spastic hemiplegia.
1995,
Pubmed
Yucesoy,
Extramuscular myofascial force transmission: experiments and finite element modeling.
2003,
Pubmed
Yucesoy,
Effects of inter- and extramuscular myofascial force transmission on adjacent synergistic muscles: assessment by experiments and finite-element modeling.
2003,
Pubmed
Zuurbier,
Changes in geometry of actively shortening unipennate rat gastrocnemius muscle.
1993,
Pubmed
van Eijden,
Heterogeneity of fiber and sarcomere length in the human masseter muscle.
1992,
Pubmed
