Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Analysis of yeast chromosomal regions carrying members of the glutamate tRNA gene family: various transposable elements are associated with them.
Hauber J
,
Stucka R
,
Krieg R
,
Feldmann H
.
???displayArticle.abstract???
We carried out an analysis on the genomic organisation of the tRNA(Glu) family in S. cerevisiae; eight clones were characterized by restriction mapping, hybridization and sequencing. These data taken together with our earlier findings show that the individual tRNA(Glu3) copies are identical only in their structural part but embedded in entirely different genomic environments. All of the tRNA genes identified here are flanked by elements such as Ty, delta, sigma, and tau. In some cases, sequences from different elements form complex patterns indicating a sophisticated history of these chromosomal regions. A novel observation is that Ty and delta in the regions analyzed are exclusively associated with the tRNA genes. The observed patterns imply that the tRNA genes mark regions of multiple transposition and subsequent excision events, but that these have occurred after the individual tRNA gene copies had been fixed in their present locations. Transcription experiments by the use of micro-injection into Xenopus oocytes suggest that the elements flanking the tRNA genes exert a modulating effect on their expression.
Andreadis,
Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.
1982, Pubmed
Andreadis,
Nucleotide sequence of yeast LEU2 shows 5'-noncoding region has sequences cognate to leucine.
1982,
Pubmed
Carle,
An electrophoretic karyotype for yeast.
1985,
Pubmed
Chisholm,
tau, a repeated DNA sequence in yeast.
1984,
Pubmed
Clark,
A yeast sigma composite element, TY3, has properties of a retrotransposon.
1988,
Pubmed
Eigel,
Structural comparison of two yeast tRNA Glu 3 genes.
1981,
Pubmed
Eigel,
Ty1 and delta elements occur adjacent to several tRNA genes in yeast.
1982,
Pubmed
Feldman,
A comparison of transcriptional linkage of tRNA cistrons in yeast and E. coli by the ultraviolet light technique.
1977,
Pubmed
Feldmann,
Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNA Glu 3 gene.
1981,
Pubmed
Gafner,
Delta sequences in the 5' non-coding region of yeast tRNA genes.
1983,
Pubmed
Genbauffe,
Tau, sigma, and delta. A family of repeated elements in yeast.
1984,
Pubmed
Genbauffe,
Induction and repression of the urea amidolyase gene in Saccharomyces cerevisiae.
1986,
Pubmed
Hauber,
Nucleotide sequence and characteristics of a Ty element from yeast.
1985,
Pubmed
Hauber,
Enhancer-like stimulation of yeast tRNA gene expression by a defined region of the Ty element micro-injected into Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Hohn,
Packaging recombinant DNA molecules into bacteriophage particles in vitro.
1977,
Pubmed
Nelböck,
Different patterns of transposable elements in the vicinity of tRNA genes in yeast: a possible clue to transcriptional modulation.
1985,
Pubmed
,
Xenbase
Rackwitz,
Analysis of cosmids using linearization by phage lambda terminase.
1985,
Pubmed
Sandmeyer,
Insertion of a repetitive element at the same position in the 5'-flanking regions of two dissimilar yeast tRNA genes.
1982,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schwartz,
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
1984,
Pubmed
Sharp,
Structure and transcription of eukaryotic tRNA genes.
1985,
Pubmed
Sprinzl,
Compilation of tRNA sequences and sequences of tRNA genes.
1987,
Pubmed
Stucka,
One member of the tRNA(Glu) gene family in yeast codes for a minor GAGtRNA(Glu) species and is associated with several short transposable elements.
1987,
Pubmed
Vieira,
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
1982,
Pubmed
Warmington,
Polymorphisms on the right arm of yeast chromosome III associated with Ty transposition and recombination events.
1987,
Pubmed
del Rey,
sigma, a repetitive element found adjacent to tRNA genes of yeast.
1982,
Pubmed
