Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Conversion of a phosphoseryl/threonyl phosphatase into a phosphotyrosyl phosphatase.
Goris J
,
Pallen CJ
,
Parker PJ
,
Hermann J
,
Waterfield MD
,
Merlevede W
.
???displayArticle.abstract???
By use of the autophosphorylated epidermal-growth-factor receptor and the synthetic peptide RRLIE-DAEY(P)AARG, representing an autophosphorylation site of the transforming protein of Rous-sarcoma virus, it is demonstrated that the phosphotyrosyl phosphatase activity of the polycation-stimulated phosphatases is substantially increased by an enzyme-directed effect of ATP or PPi. Concomitant with this increase in phosphotyrosyl phosphatase activity, the phosphorylase phosphatase activity is decreased, thus dramatically changing the substrate specificity of these enzymes. The dephosphorylation of four different phosphotyrosyl sites of the epidermal-growth-factor receptor is neither consecutive nor at random, but a preferred dephosphorylation of the P1 site over the P3 greater than P2 greater than P4 sites is observed. This phosphatase activity represents a substantial fraction of the total phosphotyrosyl phosphatase activity in the post-mitochondrial supernatant of Xenopus laevis oocytes.
Agostinis,
Dephosphorylation of phosphoproteins and synthetic phosphopeptides. Study of the specificity of the polycation-stimulated and MgATP-dependent phosphorylase phosphatases.
1987, Pubmed
Agostinis,
Dephosphorylation of phosphoproteins and synthetic phosphopeptides. Study of the specificity of the polycation-stimulated and MgATP-dependent phosphorylase phosphatases.
1987,
Pubmed
Chen,
Requirement for intrinsic protein tyrosine kinase in the immediate and late actions of the EGF receptor.
,
Pubmed
Chernoff,
Characterization of a phosphotyrosyl protein phosphatase activity associated with a phosphoseryl protein phosphatase of Mr = 95,000 from bovine heart.
1983,
Pubmed
Chou,
Human insulin receptors mutated at the ATP-binding site lack protein tyrosine kinase activity and fail to mediate postreceptor effects of insulin.
1987,
Pubmed
Downward,
Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences.
,
Pubmed
Downward,
Autophosphorylation sites on the epidermal growth factor receptor.
,
Pubmed
Ek,
Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor.
1982,
Pubmed
FISCHER,
The isolation and crystallization of rabbit skeletal muscle phosphorylase b.
1958,
Pubmed
Foulkes,
Separation of multiple phosphotyrosyl-and phosphoseryl-protein phosphatases from chicken brain.
1983,
Pubmed
Goris,
Regulation of protein phosphatase activity by the deinhibitor protein.
1984,
Pubmed
Goris,
Identification of the phosphatase deinhibitor protein phosphatases in rabbit skeletal muscle.
1986,
Pubmed
Hermann,
Modulation of the substrate specificity of the polycation-stimulated protein phosphatase from Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Honegger,
Point mutation at the ATP binding site of EGF receptor abolishes protein-tyrosine kinase activity and alters cellular routing.
1987,
Pubmed
Jacobs,
Somatomedin-C stimulates the phosphorylation of the beta-subunit of its own receptor.
1983,
Pubmed
KREBS,
The muscle phosphorylase b kinase reaction.
1958,
Pubmed
Kasuga,
Insulin stimulation of phosphorylation of the beta subunit of the insulin receptor. Formation of both phosphoserine and phosphotyrosine.
1982,
Pubmed
Li,
Characterization of a novel alkaline phosphatase activity which co-purifies with a phosphorylase (phosphoprotein) phosphatase of Mr = 35,000 cardiac muscle.
1979,
Pubmed
Li,
Purification and properties of a phosphorylase (phosphoprotein) phosphatase associated with an alkaline phosphatase of Mr 35000 from bovine adrenal cortex.
1979,
Pubmed
Li,
Co-purification of type I alkaline phosphatase and type I phosphoprotein phosphatase from various animal tissues.
1981,
Pubmed
Manalan,
Activation of calcineurin by limited proteolysis.
1983,
Pubmed
Martin,
Mechanistic aspects of the low-molecular-weight phosphatase activity of the calmodulin-activated phosphatase, calcineurin.
1986,
Pubmed
Merlevede,
Regulation of ATP-Mg-dependent protein phosphatase.
1984,
Pubmed
Petruzzelli,
Insulin activates a tyrosine-specific protein kinase in extracts of 3T3-L1 adipocytes and human placenta.
1982,
Pubmed
Ramachandran,
The interrelationship between cAMP-dependent alpha and beta subunit phosphorylation in the regulation of phosphorylase kinase activity. Studies using subunit specific phosphatases.
1987,
Pubmed
Sharma,
Use of calmodulin affinity chromatography for purification of specific calmodulin-dependent enzymes.
1983,
Pubmed
Sherr,
The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1.
1985,
Pubmed
Silberman,
Isolation and characterization of rabbit skeletal muscle protein phosphatases C-I and C-II.
1984,
Pubmed
Stern,
p185, a product of the neu proto-oncogene, is a receptorlike protein associated with tyrosine kinase activity.
1986,
Pubmed
Thom,
Rapid isolation of plasma membranes in high yield from cultured fibroblasts.
1977,
Pubmed
Ushiro,
Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
1980,
Pubmed
Waelkens,
The polycation-stimulated protein phosphatases: regulation and specificity.
1987,
Pubmed
Waelkens,
Purification and properties of polycation-stimulated phosphorylase phosphatases from rabbit skeletal muscle.
1987,
Pubmed
