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XB-ART-26942
J Microsc 1989 Feb 01;153Pt 2:223-9. doi: 10.1111/j.1365-2818.1989.tb00562.x.
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Simple three-dimensional imaging of HRP-labelled neurons with the aid of an image processor.

van der Linden JA , Terlou M , Kramer M .


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A method is described for visualizing the three-dimensional structure of horseradish peroxidase-labelled neurons in the central nervous system of Xenopus laevis tadpoles. The reconstruction is simple, and performed on a commercially available image processing system. Minimal operational skill is required for using this method. Also, various improvements were made where user's simplicity (user-friendliness), speed and required computer memory are concerned. In addition, the excellent properties of the IBAS II system, such as image filtering and image editing functions are fully available, offering a fast, clean and complete final image reconstruction.

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