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XB-ART-26651
Anal Biochem 1989 Jul 01;1801:177-80. doi: 10.1016/0003-2697(89)90108-5.
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Large scale isolation of nuclei from oocytes of Xenopus laevis.

Ruberti I , Beccari E , Bianchi E , Carnevali F .


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We describe an improvement on the procedure of Scalenghe et al. (F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa (1978) Chromosoma 66, 299-308) for the large scale isolation of nuclei from Xenopus laevis oocytes. The nuclear extract obtained was tested for its ability to transcribe a cloned Xenopus 5 S RNA gene and for the presence of nuclear factors interacting with a X. laevis ribosomal protein gene promoter. Efficiency of accurate transcription and of factor binding is comparable with that of an extract prepared from manually isolated nuclei.

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Species referenced: Xenopus laevis
Genes referenced: tbx2