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XB-ART-26387
Nucleic Acids Res 1989 Nov 25;1722:9381-94.
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Sequences preceding the minimal promoter of the Xenopus somatic 5S RNA gene increase binding efficiency for transcription factors.

Reynolds WF .


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Sequences preceding the minimal promoter play a role in the differential expression of the Xenopus somatic and oocyte-type 5S RNA genes. In this report, the somatic sequences between -32 and +37 are shown to increase transcriptional activity in microinjected embryos, yet have little to no effect in microinjected oocyte nuclei. In vitro, these sequences increase activity in whole oocyte S150 extracts, but not in oocyte nuclear extracts. In S150 extracts, these somatic sequences facilitate binding by a commonly required factor(s), other than TFIIIA, which forms a stable complex with the 5S gene. This transcriptional enhancement is also apparent in a reconstituted system using purified TFIIIA and partially purified TFIIIB and TFIIIC.

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Species referenced: Xenopus laevis
Genes referenced: bdp1 gtf3a gtf3c1

References [+] :
Andrews, Competition between Xenopus satellite I sequences and Pol III genes for stable transcription complex formation. 1984, Pubmed, Xenbase