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Functional somatostatin (SRIF, somatotropin release-inhibiting factor) receptors were expressed in Xenopus oocytes after injection of RNA isolated from the anterior pituitary tumor cell line AtT20. SRIF receptors were detected by measuring the ability of SRIF to inhibit cAMP formation stimulated by beta 2-adrenergic agonists in individual oocytes. beta 2-Adrenergic receptors (beta 2ARs) were expressed in oocytes by coinjecting RNA prepared by in vitro transcription of a beta 2AR cDNA clone with the pituitary cell RNA. Uninjected oocytes do not express detectable levels of either beta 2ARs or SRIF receptors. In oocytes coinjected with AtT20 and beta 2AR RNA, on the other hand, isoproterenol treatment led to a 2- to 3-fold increase in cAMP levels, whereas cotreatment with SRIF reduced this accumulation by 50-60%. The SRIF precursor somatostatin-28 and the cyclohexapeptide agonist MK678 also inhibited cAMP formation, whereas the biologically inactive N-terminal 14-amino acid fragment of somatostatin-28 was ineffective. The ability to detect changes in cAMP levels in individual oocytes may provide a simple procedure for the expression cloning of SRIF receptor cDNAs and other receptors functionally coupled to stimulation or inhibition of adenylate cyclase.
Axelrod,
Stress hormones: their interaction and regulation.
1984, Pubmed
Axelrod,
Stress hormones: their interaction and regulation.
1984,
Pubmed
Bonner,
Identification of a family of muscarinic acetylcholine receptor genes.
1987,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Dixon,
Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.
,
Pubmed
Epelbaum,
Somatostatin in the central nervous system: physiology and pathological modifications.
1986,
Pubmed
Jakobs,
A nucleotide regulatory site for somatostatin inhibition of adenylate cyclase in S49 lymphoma cells.
,
Pubmed
Julius,
Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
1988,
Pubmed
,
Xenbase
Kaneko,
GTP-binding proteins Gi and Go transplanted onto Xenopus oocyte by rat brain messenger RNA.
1987,
Pubmed
,
Xenbase
Kobilka,
Functional activity and regulation of human beta 2-adrenergic receptors expressed in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Kobilka,
Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.
1987,
Pubmed
,
Xenbase
Kobilka,
Chimeric alpha 2-,beta 2-adrenergic receptors: delineation of domains involved in effector coupling and ligand binding specificity.
1988,
Pubmed
Koch,
Pertussis toxin blocks both cyclic AMP-mediated and cyclic AMP-independent actions of somatostatin. Evidence for coupling of Ni to decreases in intracellular free calcium.
1985,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Lübbert,
cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Mahy,
Pertussis toxin modifies the characteristics of both the inhibitory GTP binding proteins and the somatostatin receptor in anterior pituitary tumor cells.
1988,
Pubmed
Mandarino,
Selective effects of somatostatin-14, -25 and -28 on in vitro insulin and glucagon secretion.
1981,
Pubmed
Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase
Mishina,
Location of functional regions of acetylcholine receptor alpha-subunit by site-directed mutagenesis.
,
Pubmed
,
Xenbase
Moriarty,
Functional expression of brain cholecystokinin and bombesin receptors in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Reichlin,
Somatostatin.
1983,
Pubmed
Reisine,
Pertussis toxin treatment blocks the inhibition of somatostatin and increases the stimulation by forskolin of cyclic AMP accumulation and adrenocorticotropin secretion from mouse anterior pituitary tumor cells.
1985,
Pubmed
Seamon,
Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells.
1981,
Pubmed
Smith,
Expression of rat mRNA coding for hormone-stimulated adenylate cyclase in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Snutch,
The use of Xenopus oocytes to probe synaptic communication.
1988,
Pubmed
,
Xenbase
Veber,
A super active cyclic hexapeptide analog of somatostatin.
1984,
Pubmed
White,
Mouse-Torpedo hybrid acetylcholine receptors: functional homology does not equal sequence homology.
1985,
Pubmed
,
Xenbase
Williams,
Expression of receptors for cholecystokinin and other Ca2+-mobilizing hormones in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
