Pflugers Arch 1990 Feb 01;4155:624-9.

A stretch-activated K+ channel in the basolateral membrane of Xenopus kidney proximal tubule cells.

???displayArticle.abstract???
The present study examined whether a basolateral potassium ion (K+) channel is activated by membrane-stretching in the cell-attached patch. A K+ channel of conductance of 27.5 pS was most commonly observed in the basolateral membrane of Xenopus kidney proximal tubule cells. Channel activity increased with hyperpolarizing membrane potentials [at more positive pipette potentials (Vp)]. Open probability (Po) was 0.03, 0.13, and 0.21 at Vp values of 0, 40, and 80 mV, respectively. Barium (0.1 mM) in the pipette reduced Po by 79% at a Vp of 40 mV. Application of negative hydraulic pressure (-16 to -32 cm H2O) to the pipette markedly activated outward currents (from Po = 0.01 to 0.75) at a Vp of -80 mV, but not inward currents at a Vp of 80 mV. The size of the activated outward currents (from cell to pipette) did not change by replacing chloride with gluconate in the pipette. These results indicate that a stretch-activated K+ channel exists in the basolateral membrane of proximal tubule cells. It may play an important role as a K+ exit pathway when the cell membrane is stretched (for example, by cell swelling).

???displayArticle.pubmedLink??? 2326155




References [+] :