Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Clin Exp Metastasis
1990 Jan 01;83:227-39. doi: 10.1007/bf00141254.
Show Gene links
Show Anatomy links
Adhesion of frog pronephric tumor cells to normal cells cultivated on microcarrier beads.
Tweedell KS
.
???displayArticle.abstract???
The processes of cell adhesion and active spreading were assessed between frog normal pronephric, pronephric tumor and heterologous liver cells. Confluent monolayers were developed on collagen-coated microcarrier beads, then exposed to homologous or heterologous cells and cultivated with a rotary (orbital) flask culture technique at 23 degrees C. All three cell lines attach and actively spread on the collagen-coated microcarrier beads. Secondary attachment of normal (non-transformed) proliferating cells to their confluent monolayers occurs but cell spreading is restrained. Dissociated pronephric tumor cells adhere and actively spread on the surfaces of normal pronephric cells, and eventually encapsulate them. Normal pronephric cells do not adhere readily to the cell surfaces of pronephric tumor cells grown on microcarrier beads. Tumor cells also attach, actively spread and overgrow heterologous liver cells attached to microcarrier beads. Suboptimal temperatures (17 degrees C) slow tumor cell attachment and spreading on normal cells. Colder temperature (8 degrees C) permits tumor cell attachment and adhesion to normal cell-coated beads but active cell spreading is prohibited. The same temperature retards cell spreading directly on the collagen-coated beads.
LUCKE,
Induction of metastasis of frog carcinoma by increase of environmental temperature.
1949, Pubmed
LUCKE,
Induction of metastasis of frog carcinoma by increase of environmental temperature.
1949,
Pubmed
Mareel,
Methods of study of the invasion of malignant C3H-mouse fibroblasts into embryonic chick heart in vitro.
1979,
Pubmed
Mareel,
Is invasiveness in vitro characteristic of malignant cells?
1979,
Pubmed
McKinnell,
Cytoplasmic microtubules of normal and tumor cells of the leopard frog. Temperature effects.
1984,
Pubmed
McKinnell,
Temperature-dependent metastasis of the Lucke renal carcinoma and its significance for studies on mechanisms of metastasis.
1984,
Pubmed
McKinnell,
Temperature-dependent malignant invasion in vitro by frog renal carcinoma-derived PNKT-4B cells.
1988,
Pubmed
McKinnell,
Invasion in vitro by explants of Lucke renal carcinoma cocultured with normal tissue is temperature dependent.
1986,
Pubmed
Nicolson,
Cancer metastasis. Organ colonization and the cell-surface properties of malignant cells.
1982,
Pubmed
Nicolson,
Cell surface molecules and tumor metastasis. Regulation of metastatic phenotypic diversity.
1984,
Pubmed
Nicolson,
Trans-membrane control of the receptors on normal and tumor cells. II. Surface changes associated with transformation and malignancy.
1976,
Pubmed
Ogilvie,
Temperature-dependent elaboration of collagenase by the renal adenocarcinoma of the leopard frog, Rana pipiens.
1984,
Pubmed
Raz,
Lectin-like activities associated with human and murine neoplastic cells.
1981,
Pubmed
Roth,
An assay for intercellular adhesive specificity.
1971,
Pubmed
Seppanen,
Temperature-dependent dissociation of Lucké renal adenocarcinoma cells.
1984,
Pubmed
Tweedell,
Pronephric tumour cell lines from herpesvirus-transformed cells.
1978,
Pubmed
Tweedell,
Frog kidney tumors induced by herpesvirus cultured in pronephric cells.
1974,
Pubmed
Tweedell,
Localization of glycoconjugates on the surfaces of pronephric tumor cells in vitro.
1982,
Pubmed
Tweedell,
Induced oncogenesis in developing frog kidney cells.
1967,
Pubmed
Vosbeck,
Assay of intercellular adhesiveness using cell-coated Sephadex beads as collecting particles.
1976,
Pubmed
Weisenberg,
Microtubule formation in vitro in solutions containing low calcium concentrations.
1972,
Pubmed
Wong,
Viral carcinogenesis in a pronephric cell line. An ultrastructural study.
1975,
Pubmed
de Ridder,
Adhesion of malignant and nonmalignant cells to cultured embryonic substrates.
1975,
Pubmed
