Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
cis-acting enhancement of RNA polymerase III gene expression in vitro.
Sekiguchi JA
,
Kmiec EB
.
???displayArticle.abstract???
The Xenopus laevis S-150 cell-free extract catalyzes in vitro transcription of several RNA polymerase III genes. Among these are the Xenopus 5S RNA gene (somatic type) and the Xenopus methionine tRNA gene. In this report we present an analysis of the transcriptional activity of these two genes either in trans-competition experiments or when the genes are co-localized in the same circular plasmid. In the "cis" arrangement, elevated levels of 5S and tRNA gene expression are observed, which are dependent on the relative orientation of the two genes (convergent or in tandem) and the distance between them. The results of these analyses reveal important parameters affecting the expression of juxtaposed genes.
Abraham,
Regulation of mating-type information in yeast. Negative control requiring sequences both 5' and 3' to the regulated region.
1984, Pubmed
Abraham,
Regulation of mating-type information in yeast. Negative control requiring sequences both 5' and 3' to the regulated region.
1984,
Pubmed
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Brill,
Transcription-dependent DNA supercoiling in yeast DNA topoisomerase mutants.
1988,
Pubmed
Brown,
The role of stable complexes that repress and activate eucaryotic genes.
1984,
Pubmed
,
Xenbase
Choder,
In vitro transcribed SV40 minichromosomes, as the bulk minichromosomes, have a low level of unconstrained negative supercoils.
1988,
Pubmed
Cockerill,
Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites.
1986,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Geiduschek,
Transcription by RNA polymerase III.
1988,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Hirose,
In vitro transcription of eukaryotic genes is affected differently by the degree of DNA supercoiling.
1988,
Pubmed
Huang,
Delineation of DNA sequences that are important for in vitro transcription from the adenovirus EIIa late promoter.
1988,
Pubmed
Kmiec,
Gyration is required for 5S RNA transcription from a chromatin template.
1986,
Pubmed
,
Xenbase
Kmiec,
Studies on the ATP requirements of in vitro chromatin assembly.
1989,
Pubmed
,
Xenbase
Knezetic,
Assembly of RNA polymerase II preinitiation complexes before assembly of nucleosomes allows efficient initiation of transcription on nucleosomal templates.
1988,
Pubmed
Knezetic,
The presence of nucleosomes on a DNA template prevents initiation by RNA polymerase II in vitro.
1986,
Pubmed
,
Xenbase
Liu,
Supercoiling of the DNA template during transcription.
1987,
Pubmed
Morton,
In vitro transcription of a silkworm 5S RNA gene requires an upstream signal.
1984,
Pubmed
,
Xenbase
Peck,
Transcriptionally inactive oocyte-type 5S RNA genes of Xenopus laevis are complexed with TFIIIA in vitro.
1987,
Pubmed
,
Xenbase
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Razik,
Pathways of nucleoprotein assembly on 5S RNA genes in a Xenopus oocyte S-150 extract.
1989,
Pubmed
,
Xenbase
Razvi,
A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
1983,
Pubmed
,
Xenbase
Reynolds,
Sequence differences upstream of the promoters are involved in the differential expression of the Xenopus somatic and oocyte 5S RNA genes.
1988,
Pubmed
,
Xenbase
Ruberti,
Mechanism of chromatin assembly in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Sekiguchi,
DNA superhelicity enhances the assembly of transcriptionally active chromatin in vitro.
1989,
Pubmed
,
Xenbase
Sekiguchi,
Changes in DNA topology can modulate in vitro transcription of certain RNA polymerase III genes.
1989,
Pubmed
,
Xenbase
Tabuchi,
DNA supercoiling facilitates formation of the transcription initiation complex on the fibroin gene promoter.
1988,
Pubmed
Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase
Wu,
Transcription generates positively and negatively supercoiled domains in the template.
1988,
Pubmed
