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XB-ART-25513
Biochem Biophys Res Commun 1990 Oct 30;1722:570-5.
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Expression of the murine interleukin-5 receptor on Xenopus laevis oocytes.

Devos R , Tavernier J , Plaetinck G , Van der Heyden J , Rolink A , Fiers W .


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In this study we describe the use of Xenopus laevis oocytes for the detection of mRNA coding for a murine interleukin-5 (mI15) receptor. When injected with sucrose gradient fractionated polyA+ RNA derived from the murine 115-dependent pre B cell line B13, these oocytes could specifically bind 35S-methionine labeled mI15. A size of approximately 4000 nucleotides (25S) was estimated for the mRNA corresponding to the mIL5-binding activity. This binding was not blocked by a monoclonal antibody R52 specific for the MI15-receptor, suggesting that the oocytes express a different form of this receptor.

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