Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Aleurain, originally described from its cDNA as a thiol protease [Rogers, J.C., Dean, D., and Heck, G.R. (1985). Proc. Natl. Acad. Sci. USA 82, 6512-6516], is characterized here as a glycoprotein that is targeted to a distinct vacuolar compartment in aleurone cells. Monospecific antibodies to a bacterial trpE-aleurain fusion protein were used to show that aleurain is made as a 42-kilodalton (kD) proenzyme (proaleurain) that is proteolytically processed in a post-Golgi compartment in two steps to form a 32-kD protein. The first processing step is the discrete loss of 9 kD from proaleurain to yield a 33-kD intermediate that is further processed by the gradual loss of 1 kD resulting in mature 32-kD aleurain. Using proaleurain secreted from Xenopus oocytes as a substrate, we established an in vitro system using aleurone cell extracts that correctly processes proaleurain to a stable protein that is indistinguishable from native barley aleurain as judged by partial digestion with staphylococcal V8 protease. Proaleurain is not capable of self-cleavage in the absence of aleurone cell extracts and mature aleurain appears not to participate in processing in vitro.
Ammerer,
PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.
1986, Pubmed
Ammerer,
PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.
1986,
Pubmed
Anderson,
Immunoprecipitation of proteins from cell-free translations.
1983,
Pubmed
Barrett,
Cathepsin B, Cathepsin H, and cathepsin L.
1981,
Pubmed
Chan,
Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs.
1986,
Pubmed
Chrispeels,
Regulation of reserve protein metabolism in the cotyledons of mung bean seedlings.
1976,
Pubmed
Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977,
Pubmed
Cohen,
Cloning and sequencing of papain-encoding cDNA.
1986,
Pubmed
Fuchs,
Molecular cloning and sequencing of a cDNA coding for mature human kidney cathepsin H.
1988,
Pubmed
Fuller,
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.
1989,
Pubmed
Hammerton,
Hormonal regulation of the development of protease and carboxypeptidase activities in barley aleurone layers.
1986,
Pubmed
Hara-Nishimura,
Proglobulin processing enzyme in vacuoles isolated from developing pumpkin cotyledons.
1987,
Pubmed
Hasilik,
Enzymatic phosphorylation of lysosomal enzymes in the presence of UDP-N-acetylglucosamine. Absence of the activity in I-cell fibroblasts.
1981,
Pubmed
Hoffman,
Dystrophin: the protein product of the Duchenne muscular dystrophy locus.
1987,
Pubmed
Hurn,
Production of reagent antibodies.
1980,
Pubmed
Ishidoh,
Molecular cloning and sequencing of cDNA for rat cathepsin H. Homology in pro-peptide regions of cysteine proteinases.
1987,
Pubmed
Klionsky,
Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase.
1989,
Pubmed
Koehler,
Purification and characterization of gibberellic Acid-induced cysteine endoproteases in barley aleurone layers.
1988,
Pubmed
Koehler,
Hormonal regulation, processing, and secretion of cysteine proteinases in barley aleurone layers.
1990,
Pubmed
Kornfeld,
Assembly of asparagine-linked oligosaccharides.
1985,
Pubmed
Lippincott-Schwartz,
Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER.
1989,
Pubmed
Marciniszyn,
Mechanism of intramolecular activation of pepsinogen. Evidence for an intermediate delta and the involvement of the active site of pepsin in the intramolecular activation of pepsinogen.
1976,
Pubmed
Mechler,
Maturation of vacuolar (lysosomal) enzymes in yeast: proteinase yscA and proteinase yscB are catalysts of the processing and activation event of carboxypeptidase yscY.
1987,
Pubmed
Mechler,
Biogenesis of the yeast lysosome (vacuole): biosynthesis and maturation of proteinase yscB.
1988,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Nishimura,
Intracellular transport and processing of lysosomal cathepsin H.
1987,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Rogers,
Aleurain: a barley thiol protease closely related to mammalian cathepsin H.
1985,
Pubmed
Sanny,
Conversion of pepsinogen to pepsin. Further evidence for intramolecular and pepsin-catalyzed activation.
1975,
Pubmed
Smith,
Activity and deletion analysis of recombinant human cathepsin L expressed in Escherichia coli.
1989,
Pubmed
Sonnewald,
Expression of mutant patatin protein in transgenic tobacco plants: role of glycans and intracellular location.
1990,
Pubmed
Tartakoff,
Perturbation of vesicular traffic with the carboxylic ionophore monensin.
1983,
Pubmed
Vigil,
Effect of gibberellic Acid and actinomycin d on the formation and distribution of rough endoplasmic reticulum in barley aleurone cells.
1973,
Pubmed
Voelker,
In vitro mutated phytohemagglutinin genes expressed in tobacco seeds: role of glycans in protein targeting and stability.
1989,
Pubmed
Whittier,
Nucleotide sequence analysis of alpha-amylase and thiol protease genes that are hormonally regulated in barley aleurone cells.
1987,
Pubmed
Woolford,
The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.
1986,
Pubmed
Yomo,
Hormonal control of a secretory tissue.
1971,
Pubmed
von Figura,
Lysosomal enzymes and their receptors.
1986,
Pubmed