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Naunyn Schmiedebergs Arch Pharmacol
1991 Mar 01;3433:334-6.
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Expression of the neuronal noradrenaline transporter in Xenopus laevis oocytes.
Coppeneur D
,
Lingen B
,
Sanders G
,
Dabauvalle MC
,
Bönisch H
.
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RNA isolated from both rat phaeochromocytoma (PC12) cells and bovine adrenal medulla was size-selected and injected into oocytes of Xenopus laevis. Expression of the neuronal noradrenaline transporter was assayed 3 days after injection by measuring the nisoxetine-sensitive noradrenaline uptake. A 2 kilobase fraction of either total RNA derived from both tissues or of polyadenylated mRNA obtained from bovine adrenal medulla caused Na(+)-dependent transport of 3H-noradrenaline in the injected oocytes.
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