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Receptors of the serotonin 1C subtype expressed from cloned DNA mediate the closing of K+ membrane channels encoded by brain mRNA.
Panicker MM
,
Parker I
,
Miledi R
.
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The modulation of K+ channels by serotonin (5-HT) receptors was studied by coinjecting Xenopus oocytes with mRNA transcribed in vitro from a cloned 5-HT 1C subtype (5-HT1C) receptor gene, together with size-fractionated mRNA isolated from rat cerebral cortex that expresses K+ channels. After intracellular loading with EGTA to block Ca2(+)-dependent chloride currents, these oocytes responded to 5-HT with an inward current associated with a decrease in membrane conductance. Membrane current responses were small or absent in oocytes injected with either mRNA alone. We conclude that 5-HT1C receptors are able to cause the closing of a class of K+ channels expressed by cortex mRNA in a Ca2(+)-independent manner. The coupling between the receptors and channels appears to be mediated by the inositol phospholipid second messenger pathway, since activation of this pathway by application of serum evoked a similar closing current.
Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986, Pubmed,
Xenbase
Dascal,
Involvement of a GTP-binding protein in mediation of serotonin and acetylcholine responses in Xenopus oocytes injected with rat brain messenger RNA.
1986,
Pubmed
,
Xenbase
Gundersen,
Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Julius,
Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
1988,
Pubmed
,
Xenbase
Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase
Luthe,
A simple technique for the preparation and storage of sucrose gradients.
1983,
Pubmed
Lübbert,
cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Miledi,
Chloride current induced by injection of calcium into Xenopus oocytes.
1984,
Pubmed
,
Xenbase
Miledi,
Membrane currents elicited by prostaglandins, atrial natriuretic factor and oxytocin in follicle-enclosed Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Nomura,
Inositol phosphate formation and chloride current responses induced by acetylcholine and serotonin through GTP-binding proteins in Xenopus oocyte after injection of rat brain messenger RNA.
1987,
Pubmed
,
Xenbase
Oron,
Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes.
,
Pubmed
,
Xenbase
Parker,
Activation of a common effector system by different brain neurotransmitter receptors in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Parker,
Serotonin receptors expressed in Xenopus oocytes by mRNA from brain mediate a closing of K+ membrane channels.
1990,
Pubmed
,
Xenbase
Parker,
Intracellular Ca2+-dependent and Ca2+-independent responses of rat brain serotonin receptors transplanted to Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Takahashi,
Rat brain serotonin receptors in Xenopus oocytes are coupled by intracellular calcium to endogenous channels.
1987,
Pubmed
,
Xenbase
Tigyi,
A serum factor that activates the phosphatidylinositol phosphate signaling system in Xenopus oocytes.
1990,
Pubmed
,
Xenbase