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Regulation of pp90rsk phosphorylation and S6 phosphotransferase activity in Swiss 3T3 cells by growth factor-, phorbol ester-, and cyclic AMP-mediated signal transduction.
Chen RH
,
Chung J
,
Blenis J
.
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Somatic cell homologs to the Xenopus laevis S6 protein kinases (referred to collectively as pp90rsk) have recently been identified and partially characterized. Here we examine alterations in pp90rsk phosphorylation and S6 phosphotransferase activity in response to regulators of multiple signal transduction systems: purified growth factors, phorbol ester, changes in cyclic AMP (cAMP) levels, and sodium vanadate. All reagents tested increased pp90rsk serine and threonine phosphorylation, but only those agents that regulate cell proliferation and sodium vanadate activated its S6 kinase activity. In addition to the cAMP-stimulated phosphorylation of pp90rsk, a simple correlation between the extent of growth-regulated pp90rsk phosphorylation and S6 phosphotransferase activity was not observed. Quantitative phosphorylation of pp90rsk continued to increase after its S6 kinase activity began its return towards basal levels. However, a close correlation between the appearance and disappearance of a slow-mobility form of phosphorylated pp90rsk (by electrophoresis) and pp90rsk activity was observed. In addition, pp90rsk was regulated by both protein kinase C-independent and -dependent signaling mechanisms. The extent of protein kinase C participation, however, varied depending on which growth factor receptor was activated. Furthermore, growth factor-specific differences in the temporal regulation of pp90rsk S6 phosphotransferase activity were also observed. These results support the notion that the complex regulation of the rsk gene product constitutes one of the primary responses of animal cells to mitogenic signals.
Alcorta,
Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase.
1989, Pubmed,
Xenbase
Alcorta,
Sequence and expression of chicken and mouse rsk: homologs of Xenopus laevis ribosomal S6 kinase.
1989,
Pubmed
,
Xenbase
Berridge,
Inositol phosphates and cell signalling.
1989,
Pubmed
Blenis,
Role of second messengers in regulating the growth-associated S6 protein kinase activity.
1987,
Pubmed
Blenis,
Regulation of a ribosomal protein S6 kinase activity by the Rous sarcoma virus transforming protein, serum, or phorbol ester.
1985,
Pubmed
Blenis,
Stimulation of ribosomal protein S6 kinase activity by pp60v-src or by serum: dissociation from phorbol ester-stimulated activity.
1986,
Pubmed
Blenis,
Identification of a ribosomal protein S6 kinase regulated by transformation and growth-promoting stimuli.
1987,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Chen,
Identification of Xenopus S6 protein kinase homologs (pp90rsk) in somatic cells: phosphorylation and activation during initiation of cell proliferation.
1990,
Pubmed
,
Xenbase
Chung,
Coordinate regulation of pp90rsk and a distinct protein-serine/threonine kinase activity that phosphorylates recombinant pp90rsk in vitro.
1991,
Pubmed
Cooper,
Detection and quantification of phosphotyrosine in proteins.
1983,
Pubmed
Erikson,
Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.
1987,
Pubmed
,
Xenbase
Erikson,
A protein kinase from Xenopus eggs specific for ribosomal protein S6.
1985,
Pubmed
,
Xenbase
Erikson,
In vivo phosphorylation and activation of ribosomal protein S6 kinases during Xenopus oocyte maturation.
1989,
Pubmed
,
Xenbase
Gregory,
An insulin-stimulated ribosomal protein S6 kinase from rabbit liver.
1989,
Pubmed
Halsey,
Protein kinase C in fibroblasts. Characteristics of its intracellular location during growth and after exposure to phorbol esters and other mitogens.
1987,
Pubmed
Hanocq-Quertier,
Phosphorylation of ribosomal proteins during maturation of Xenopus laevis oocytes.
1981,
Pubmed
,
Xenbase
Jenö,
Identification and characterization of a mitogen-activated S6 kinase.
1988,
Pubmed
Jones,
A Xenopus ribosomal protein S6 kinase has two apparent kinase domains that are each similar to distinct protein kinases.
1988,
Pubmed
,
Xenbase
Kozma,
A stimulated S6 kinase from rat liver: identity with the mitogen activated S6 kinase of 3T3 cells.
1989,
Pubmed
Kozma,
Unmasking a growth factor/oncogene-activated S6 phosphorylation cascade.
1989,
Pubmed
Luttrell,
Augmented mitogenic responsiveness to epidermal growth factor in murine fibroblasts that overexpress pp60c-src.
1988,
Pubmed
Nemenoff,
An S6 kinase activated during liver regeneration is related to the insulin-stimulated S6 kinase in H4 hepatoma cells.
1988,
Pubmed
Nielsen,
Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Novak-Hofer,
Role of extracellular electrolytes in the activation of ribosomal protein S6 kinase by epidermal growth factor, insulin-like growth factor 1, and insulin in ZR-75-1 cells.
1988,
Pubmed
Pelech,
Rapid microassay for protein kinase C translocation in Swiss 3T3 cells.
1986,
Pubmed
Pelech,
Mitogen-activated S6 kinase is stimulated via protein kinase C-dependent and independent pathways in Swiss 3T3 cells.
1987,
Pubmed
Rink,
Calcium signalling in non-excitable cells: notes on oscillations and store refilling.
1989,
Pubmed
Stith,
The effect of insulin on intracellular ph and ribosomal protein S6 phosphorylation in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase
Sweet,
Two distinct enzymes contribute to biphasic S6 phosphorylation in serum-stimulated chicken embryo fibroblasts.
1990,
Pubmed
,
Xenbase
Sweet,
Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts.
1990,
Pubmed
,
Xenbase
Tabarini,
Purification of a bovine liver S6 kinase.
1987,
Pubmed
Vik,
Coinfection of insect cells with recombinant baculovirus expressing pp60v-src results in the activation of a serine-specific protein kinase pp90rsk.
1990,
Pubmed
,
Xenbase