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Proc Natl Acad Sci U S A
2005 Jan 18;1023:651-5. doi: 10.1073/pnas.0408939102.
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Prion protein NMR structures of chickens, turtles, and frogs.
Calzolai L
,
Lysek DA
,
Pérez DR
,
Güntert P
,
Wüthrich K
.
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The NMR structures of the recombinant prion proteins from chicken (Gallus gallus; chPrP), the red-eared slider turtle (Trachemys scripta; tPrP), and the African clawed frog (Xenopus laevis; xlPrP) are presented. The amino acid sequences of these prion proteins show approximately 30% identity with mammalian prion proteins. All three species form the same molecular architecture as mammalian PrPC, with a long, flexibly disordered tail attached to the N-terminal end of a globular domain. The globular domain in chPrP and tPrP contains three alpha-helices, one short 3(10)-helix, and a short antiparallel beta-sheet. In xlPrP, the globular domain includes three alpha-helices and a somewhat longer beta-sheet than in the other species. The spatial arrangement of these regular secondary structures coincides closely with that of the globular domain in mammalian prion proteins. Based on the low sequence identity to mammalian PrPs, comparison of chPrP, tPrP, and xlPrP with mammalian PrPC structures is used to identify a set of essential amino acid positions for the preservation of the same PrPC fold in birds, reptiles, amphibians, and mammals. There are additional conserved residues without apparent structural roles, which are of interest for the ongoing search for physiological functions of PrPC in healthy organisms.
Bairoch,
The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000.
2000, Pubmed
Bairoch,
The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000.
2000,
Pubmed
Bartels,
The program XEASY for computer-supported NMR spectral analysis of biological macromolecules.
1995,
Pubmed
Büeler,
Mice devoid of PrP are resistant to scrapie.
1993,
Pubmed
Calzolai,
NMR structures of three single-residue variants of the human prion protein.
2000,
Pubmed
Gabriel,
Molecular cloning of a candidate chicken prion protein.
1992,
Pubmed
Gauczynski,
The 37-kDa/67-kDa laminin receptor acts as the cell-surface receptor for the cellular prion protein.
2001,
Pubmed
Gilch,
Recognition of lumenal prion protein aggregates by post-ER quality control mechanisms is mediated by the preoctarepeat region of PrP.
2004,
Pubmed
Gossert,
Prion protein NMR structures of elk and of mouse/elk hybrids.
2005,
Pubmed
Graner,
Cellular prion protein binds laminin and mediates neuritogenesis.
2000,
Pubmed
Güntert,
Torsion angle dynamics for NMR structure calculation with the new program DYANA.
1997,
Pubmed
Haire,
The crystal structure of the globular domain of sheep prion protein.
2004,
Pubmed
Harris,
A prion-like protein from chicken brain copurifies with an acetylcholine receptor-inducing activity.
1991,
Pubmed
Harris,
Cellular biology of prion diseases.
1999,
Pubmed
Hegde,
A transmembrane form of the prion protein in neurodegenerative disease.
1998,
Pubmed
Herrmann,
Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA.
2002,
Pubmed
Hornemann,
NMR structure of the bovine prion protein isolated from healthy calf brains.
2004,
Pubmed
James,
Solution structure of a 142-residue recombinant prion protein corresponding to the infectious fragment of the scrapie isoform.
1997,
Pubmed
Koradi,
MOLMOL: a program for display and analysis of macromolecular structures.
1996,
Pubmed
Luginbühl,
The new program OPAL for molecular dynamics simulations and energy refinements of biological macromolecules.
1996,
Pubmed
Lysek,
Prion protein interaction with the C-terminal SH3 domain of Grb2 studied using NMR and optical spectroscopy.
2004,
Pubmed
Lysek,
Prion protein NMR structures of cats, dogs, pigs, and sheep.
2005,
Pubmed
Lysek,
NMR assignment of the chicken prion protein fragments chPrP(128-242) and chPrP(25-242).
2004,
Pubmed
López Garcia,
NMR structure of the bovine prion protein.
2000,
Pubmed
Nunziante,
Essential role of the prion protein N terminus in subcellular trafficking and half-life of cellular prion protein.
2003,
Pubmed
,
Xenbase
Oesch,
A cellular gene encodes scrapie PrP 27-30 protein.
1985,
Pubmed
Prusiner,
Prions.
1998,
Pubmed
Rieger,
The human 37-kDa laminin receptor precursor interacts with the prion protein in eukaryotic cells.
1997,
Pubmed
Riek,
NMR structure of the mouse prion protein domain PrP(121-231).
1996,
Pubmed
Riek,
NMR characterization of the full-length recombinant murine prion protein, mPrP(23-231).
1997,
Pubmed
Simonic,
cDNA cloning of turtle prion protein.
2000,
Pubmed
Spielhaupter,
PrPC directly interacts with proteins involved in signaling pathways.
2001,
Pubmed
Stewart,
Most pathogenic mutations do not alter the membrane topology of the prion protein.
2001,
Pubmed
Strumbo,
Molecular cloning of the cDNA coding for Xenopus laevis prion protein.
2001,
Pubmed
,
Xenbase
Wopfner,
Analysis of 27 mammalian and 9 avian PrPs reveals high conservation of flexible regions of the prion protein.
1999,
Pubmed
Zahn,
Human prion proteins expressed in Escherichia coli and purified by high-affinity column refolding.
1997,
Pubmed
Zahn,
NMR solution structure of the human prion protein.
2000,
Pubmed
van Rheede,
Molecular evolution of the mammalian prion protein.
2003,
Pubmed
