Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Regulation of a double-stranded RNA modification activity in human cells.
Morrissey LM
,
Kirkegaard K
.
???displayArticle.abstract???
A double-stranded RNA (dsRNA)-specific modification activity from Xenopus oocytes and human cells dsRNA modifier) converts adenosine residues present in dsRNA to inosines. The function of the dsRNA modifier is unknown, although it has been suggested that it may be part of the cellular antiviral response. We investigated the relationship between the activity of the dsRNA modifier, viral infection, and the antiviral response in human cells induced by poly(rI)-poly(rC) [poly(I.C)] treatment. We found, unexpectedly, that treatment of HeLa cells with poly(I.C) or other dsRNA molecules resulted in the dramatic inhibition of the dsRNA modifier. Mixing experiments, reconstruction experiments, and pretreatment of extracts with RNases indicated that inhibition of the dsRNA modifier did not result from the continued presence of a soluble inhibitor such as dsRNA) in the in vitro modification reactions. Treatment of cells with cyclohexamide or dactinomycin simultaneously with the poly(I.C) demonstrated that in vivo inhibition of the dsRNA modifier did not require new transcription or translation. The dsRNA modification activity was also substantially inhibited in cells infected with poliovirus and was slightly inhibited in cells infected with adenovirus. The inhibition of the dsRNA modifier during the antiviral state is thus not consistent with an antiviral function, and instead suggests another cellular function for dsRNA modification.
BASILIO,
Synthetic polynucleotides and the amino acid code. V.
1962, Pubmed
BASILIO,
Synthetic polynucleotides and the amino acid code. V.
1962,
Pubmed
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987,
Pubmed
,
Xenbase
Bass,
Biased hypermutation of viral RNA genomes could be due to unwinding/modification of double-stranded RNA.
1989,
Pubmed
Bass,
An unwinding activity that covalently modifies its double-stranded RNA substrate.
1988,
Pubmed
,
Xenbase
Bernstein,
Poliovirus mutant that does not selectively inhibit host cell protein synthesis.
1985,
Pubmed
Black,
The cellular 68,000-Mr protein kinase is highly autophosphorylated and activated yet significantly degraded during poliovirus infection: implications for translational regulation.
1989,
Pubmed
Cattaneo,
Biased hypermutation and other genetic changes in defective measles viruses in human brain infections.
1988,
Pubmed
Cattaneo,
Mutated and hypermutated genes of persistent measles viruses which caused lethal human brain diseases.
1989,
Pubmed
Enoch,
Activation of the human beta-interferon gene requires an interferon-inducible factor.
1986,
Pubmed
Fan,
Two different virus-inducible elements are required for human beta-interferon gene regulation.
1989,
Pubmed
Friedman,
Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells.
1984,
Pubmed
Hall,
Evidence for a novel signal transduction pathway activated by platelet-derived growth factor and by double-stranded RNA.
1989,
Pubmed
Icho,
The double-stranded RNA genome of yeast virus L-A encodes its own putative RNA polymerase by fusing two open reading frames.
1989,
Pubmed
Jones,
Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
1979,
Pubmed
Kimelman,
An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Kirkegaard,
Conditional poliovirus mutants made by random deletion mutagenesis of infectious cDNA.
1990,
Pubmed
Kitajewski,
Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2 alpha kinase.
1986,
Pubmed
Kitamura,
Primary structure, gene organization and polypeptide expression of poliovirus RNA.
1981,
Pubmed
Lamb,
RNA structure. Unwinding with a vengeance.
1989,
Pubmed
Lenardo,
The involvement of NF-kappa B in beta-interferon gene regulation reveals its role as widely inducible mediator of signal transduction.
1989,
Pubmed
Lenardo,
NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control.
1989,
Pubmed
Lengyel,
Biochemistry of interferons and their actions.
1982,
Pubmed
Lockard,
Mapping tRNA structure in solution using double-strand-specific ribonuclease V1 from cobra venom.
1981,
Pubmed
Luo,
A specific base transition occurs on replicating hepatitis delta virus RNA.
1990,
Pubmed
Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed
O'Malley,
A mechanism for the control of protein synthesis by adenovirus VA RNAI.
1986,
Pubmed
O'Neill,
Inhibition of translation in cells infected with a poliovirus 2Apro mutant correlates with phosphorylation of the alpha subunit of eucaryotic initiation factor 2.
1989,
Pubmed
Pestka,
Interferons and their actions.
1987,
Pubmed
Racaniello,
Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
1981,
Pubmed
Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
Pubmed
,
Xenbase
Reich,
Transcription of interferon-stimulated genes is induced by adenovirus particles but is suppressed by E1A gene products.
1988,
Pubmed
Reich,
Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element.
1987,
Pubmed
Schneider,
Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.
1985,
Pubmed
Siekierka,
Translational control by adenovirus: lack of virus-associated RNAI during adenovirus infection results in phosphorylation of initiation factor eIF-2 and inhibition of protein synthesis.
1985,
Pubmed
Sonenberg,
Regulation of translation by poliovirus.
1987,
Pubmed
Tiwari,
Gene induction by interferons and double-stranded RNA: selective inhibition by 2-aminopurine.
1988,
Pubmed
Wagner,
Cell cycle expression of RNA duplex unwindase activity in mammalian cells.
1988,
Pubmed
,
Xenbase
Wagner,
A double-stranded RNA unwinding activity introduces structural alterations by means of adenosine to inosine conversions in mammalian cells and Xenopus eggs.
1989,
Pubmed
,
Xenbase
Weissmann,
RNA editing. Sometimes an editor makes sense.
1990,
Pubmed
Wong,
Generalized and localized biased hypermutation affecting the matrix gene of a measles virus strain that causes subacute sclerosing panencephalitis.
1989,
Pubmed
Yan,
Signal transduction pathways in the induction of 2',5'-oligoadenylate synthetase gene expression by interferon alpha/beta.
1989,
Pubmed
Zinn,
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
1983,
Pubmed
Zullo,
Platelet-derived growth factor and double-stranded ribonucleic acids stimulate expression of the same genes in 3T3 cells.
1985,
Pubmed
