Eur J Pharmacol 1991 Aug 06;2002-3:305-10. doi: 10.1016/0014-2999(91)90587-g.

Biphasic effects of mianserin and desipramine on serotonin-evoked current and Cl- efflux in Xenopus oocytes.

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Serotonin (5-HT, 1 microM) elicited two phases of Cl- inward current in Xenopus oocytes injected with rat brain mRNA: a transient current (T-current), which was generated rapidly (within 1 min), and a sustained current (S-current), which persisted for 10 min. Each type of 5-HT-evoked response was time-dependent after mRNA injection. The T-current was generated at 20-30 h and the S-current at 30-40 h. Although mianserin at 0.1 microM completely inhibited the T-current, 10 microM mianserin was required to suppress the S-current. 5-HT also caused Cl- efflux from oocytes preloaded with 36Cl-. Cl- efflux during 1 min, corresponding to the T-current, was inhibited by 0.1 microM mianserin. A higher concentration of mianserin (10 microM) was required to block the efflux for 10 min, corresponding to the S-current, as well as the current response. Desipramine selectively inhibited the T-current and Cl- efflux for 1 min. The mechanisms underlying the different sensitivity to mianserin of oocytes injected with rat brain mRNA are discussed.

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