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XB-ART-24570
J Biochem 1991 Sep 01;1103:412-6.
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Isolation and characterization of a monosialosylgangliopentaosyl ceramide from Xenopus laevis oocyte.

Hidari K , Itonori S , Sanai Y , Ohashi M , Kasama T , Nagai Y .


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A monosialosylgangliopentaosyl ceramide was isolated from Xenopus laevis oocytes. It represented 5.8% of the total acidic glycosphingolipids. From the results of sugar-composition analysis, enzymatic hydrolysis, permethylation analysis, and negative ion fast atom bombardment mass spectrometry, the structure of the ganglioside was determined to be as follows: [sequence: see text] The predominant species of fatty acids were alpha-hydroxy fatty acids, h22:0, h24:0, and h24:1. The long chain bases of this ganglioside consisted mainly of d18:1 sphingosine and phytosphingosine. Other acidic glycolipids were also characterized. The most abundant component of acidic glycolipids was sulfatide, which represented 85.7% of the total acidic glycolipid mixture. GM3, GM2, GM1a, and GD1a were also detected.

???displayArticle.pubmedLink??? 1769969
???displayArticle.link??? J Biochem