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Adrenocorticotropin receptors: functional expression from rat adrenal mRNA in Xenopus laevis oocytes.
Mertz LM
,
Catt KJ
.
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The adrenocorticotropin (ACTH) receptor, which binds corticotropin and stimulates adenylate cyclase and steroidogenesis in adrenocortical cells, was expressed in Xenopus laevis oocytes microinjected with rat adrenal poly(A)+ RNA. Expression of the ACTH receptor in individual stage 5 and 6 oocytes was monitored by radioimmunoassay of ligand-stimulated cAMP production. Injection of 5-40 ng of adrenal mRNA caused dose-dependent increases in ACTH-responsive cAMP production. These were detected at 48 h and reached a maximum 72 h after microinjection of 20-40 ng of adrenal mRNA. In response to 1 microM ACTH, total cAMP production increased within 2.5 min and reached half-maximal and maximal levels (5-fold greater than basal) at 10 and 75 min, respectively, and then remained elevated for up to 5 h. Extracellular cAMP levels were much lower but showed prominent linear increases from almost undetectable levels, with 70- and 150-fold increases evident at 1 and 2 h, respectively. The half-maximal concentration (ED50) for stimulation of cAMP formation was 5 x 10(-8) M ACTH-(1-24); the ED50 for ACTH-(1-17) was 5 x 10(-7) M, and no response was observed with ACTH-(1-10). Size fractionation of rat adrenal poly(A)+ RNA by sucrose density-gradient centrifugation revealed that mRNA encoding the ACTH receptor was present in the 1.1- to 2.0-kilobase fraction. These data indicate that ACTH receptors can be expressed from adrenal mRNA in Xenopus oocytes and are fully functional in terms of ligand specificity and signal generation. The extracellular cAMP response to ACTH is a sensitive and convenient index of receptor expression. This system should permit more complete characterization and expression cloning of the ACTH receptor.
Buckley,
Characterization of corticotropin receptors on adrenocortical cells.
1981, Pubmed
Buckley,
Characterization of corticotropin receptors on adrenocortical cells.
1981,
Pubmed
Cheitlin,
The role of extracellular calcium in corticotropin-stimulated steroidogenesis.
1985,
Pubmed
Clarke,
Differential expression of functional adrenocorticotropic hormone receptors by subpopulations of lymphocytes.
1989,
Pubmed
Garren,
Studies on the role of protein synthesis in the regulation of corticosterone production by adrenocorticotropic hormone in vivo.
1965,
Pubmed
Grahame-Smith,
Adenosine 3',5'-monophosphate as the intracellular mediator of the action of adrenocorticotropic hormone on the adrenal cortex.
1967,
Pubmed
Greenfield,
Xenopus oocyte K+ current. II. Adenylyl cyclase-linked receptors on follicle cells.
1990,
Pubmed
,
Xenbase
Hofmann,
Identification of a protein in adrenal particulates that binds adrenocorticotropin specifically and with high affinity.
1988,
Pubmed
Horiuchi,
Expression of adenylate cyclase-coupled osseous parathyroid hormone and parathyroid hormone-like peptide receptors in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Kobilka,
Functional activity and regulation of human beta 2-adrenergic receptors expressed in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Krupinski,
Adenylyl cyclase amino acid sequence: possible channel- or transporter-like structure.
1989,
Pubmed
Lefkowitz,
ACTH-receptor interaction in the adrenal: a model for the initial step in the action of hormones that stimulate adenyl cyclase.
1971,
Pubmed
Londos,
Multiple inhibitory and activating effects of nucleotides and magnesium on adrenal adenylate cyclase.
1975,
Pubmed
Masu,
cDNA cloning of bovine substance-K receptor through oocyte expression system.
,
Pubmed
,
Xenbase
McIlhinney,
Studies on the binding of 125I-labelled corticotrophin to isolated rat adrenocortical cells.
1975,
Pubmed
McIntosh,
Coupling of inositol phospholipid hydrolysis to peptide hormone receptors expressed from adrenal and pituitary mRNA in Xenopus laevis oocytes.
1987,
Pubmed
,
Xenbase
Mertz,
The kinetics of steroidogenesis activator polypeptide in the rat adrenal cortex. Effects of adrenocorticotropin, cyclic adenosine 3':5'-monophosphate, cycloheximide, and circadian rhythm.
1989,
Pubmed
Mizuno,
Affinity labeling of ACTH receptors in bovine adrenal cortex membranes.
1989,
Pubmed
Pedersen,
Steroidogenesis-activator polypeptide isolated from a rat Leydig cell tumor.
1987,
Pubmed
Penhoat,
Corticotropin positively regulates its own receptors and cAMP response in cultured bovine adrenal cells.
1989,
Pubmed
Rae,
Mutations in cyclic AMP-dependent protein kinase and corticotropin (ACTH)-sensitive adenylate cyclase affect adrenal steroidogenesis.
1979,
Pubmed
Ramachandran,
Photoaffinity labeling of corticotropin receptors.
1980,
Pubmed
Sala,
Adrenocorticotropin action in isolated adrenal cells. The intermediate role of cyclic AMP in stimulation of corticosterone synthesis.
1979,
Pubmed
Sandberg,
Calcium mobilization by angiotensin II and neurotransmitter receptors expressed in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Simpson,
Regulation of the synthesis of steroidogenic enzymes in adrenal cortical cells by ACTH.
1988,
Pubmed
Smith,
Expression of rat mRNA coding for hormone-stimulated adenylate cyclase in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Spindel,
Cloning and functional characterization of a complementary DNA encoding the murine fibroblast bombesin/gastrin-releasing peptide receptor.
1990,
Pubmed
,
Xenbase