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Mol Cell Biol
1991 Oct 01;1110:5154-63. doi: 10.1128/mcb.11.10.5154-5163.1991.
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Retrovirus-induced insertional mutagenesis: mechanism of collagen mutation in Mov13 mice.
Barker DD
,
Wu H
,
Hartung S
,
Breindl M
,
Jaenisch R
.
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The Mov13 mouse strain carries a mutation in the alpha 1(I) procollagen gene which is due to the insertion of a Moloney murine leukemia provirus into the first intron. This insertion results in the de novo methylation of the provirus and flanking DNA, the alteration of chromatin structure, and the transcriptional inactivity of the collagen promoter. To address the mechanism of mutagenesis, we reintroduced a cloned and therefore demethylated version of the Mov13 mutant allele into mouse fibroblasts. The transfected gene was not transcribed, indicating that the transcriptional defect was not due to the hypermethylation. Rather, this result strongly suggests that the mutation is due to the displacement or disruption of cis-acting regulatory DNA sequences within the first intron. We also constructed a Mov13 variant allele containing a single long terminal repeat instead of the whole provirus. This construct also failed to express mRNA, indicating that the Mov13 mutation does not revert by provirus excision as has been observed for other retrovirus-induced mutations.
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977, Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Bornstein,
Interactions between the promoter and first intron are involved in transcriptional control of alpha 1(I) collagen gene expression.
1988,
Pubmed
Bornstein,
The first intron of the alpha 1(I) collagen gene contains several transcriptional regulatory elements.
1988,
Pubmed
Breindl,
Retrovirus-induced lethal mutation in collagen I gene of mice is associated with an altered chromatin structure.
1984,
Pubmed
Chan,
Retrovirus-induced interference with collagen I gene expression in Mov13 fibroblasts is maintained in the absence of DNA methylation.
1991,
Pubmed
,
Xenbase
Copeland,
Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs.
1983,
Pubmed
Harbers,
Insertion of retrovirus into the first intron of alpha 1(I) collagen gene to embryonic lethal mutation in mice.
1984,
Pubmed
Hartung,
Retrovirus insertion inactivates mouse alpha 1(I) collagen gene by blocking initiation of transcription.
,
Pubmed
Jaenisch,
Chromosomal position and activation of retroviral genomes inserted into the germ line of mice.
1981,
Pubmed
Jaenisch,
Germline integration of moloney murine leukemia virus at the Mov13 locus leads to recessive lethal mutation and early embryonic death.
1983,
Pubmed
Jenkins,
Dilute (d) coat colour mutation of DBA/2J mice is associated with the site of integration of an ecotropic MuLV genome.
1981,
Pubmed
Jähner,
Chromosomal position and specific demethylation in enhancer sequences of germ line-transmitted retroviral genomes during mouse development.
1985,
Pubmed
Jähner,
Integration of Moloney leukaemia virus into the germ line of mice: correlation between site of integration and virus activation.
1980,
Pubmed
Jähner,
De novo methylation and expression of retroviral genomes during mouse embryogenesis.
1982,
Pubmed
Jähner,
Retrovirus-induced de novo methylation of flanking host sequences correlates with gene inactivity.
,
Pubmed
Kratochwil,
Retrovirus-induced insertional mutation in Mov13 mice affects collagen I expression in a tissue-specific manner.
1989,
Pubmed
Lemischka,
The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element.
1982,
Pubmed
Lenardo,
NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control.
1989,
Pubmed
Levis,
Effects of transposable element insertions on RNA encoded by the white gene of Drosophila.
1984,
Pubmed
Liau,
Coordinate regulation of the levels of type III and type I collagen mRNA in most but not all mouse fibroblasts.
1985,
Pubmed
Liska,
A highly conserved intronic sequence is involved in transcriptional regulation of the alpha 1(I) collagen gene.
1990,
Pubmed
Loh,
Proviral sequences that restrict retroviral expression in mouse embryonal carcinoma cells.
1987,
Pubmed
Löhler,
Embryonic lethal mutation in mouse collagen I gene causes rupture of blood vessels and is associated with erythropoietic and mesenchymal cell death.
1984,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Modolell,
Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element.
1983,
Pubmed
Niwa,
Independent mechanisms involved in suppression of the Moloney leukemia virus genome during differentiation of murine teratocarcinoma cells.
1983,
Pubmed
Parkhurst,
Forked, gypsys, and suppressors in Drosophila.
1985,
Pubmed
Paro,
Molecular analysis of large transposable elements carrying the white locus of Drosophila melanogaster.
1983,
Pubmed
Rippe,
Regulatory elements in the 5'-flanking region and the first intron contribute to transcriptional control of the mouse alpha 1 type I collagen gene.
1989,
Pubmed
Rossi,
Identification of a cell-specific transcriptional enhancer in the first intron of the mouse alpha 2 (type I) collagen gene.
1987,
Pubmed
Rossouw,
DNA sequences in the first intron of the human pro-alpha 1(I) collagen gene enhance transcription.
1987,
Pubmed
,
Xenbase
Schmidt,
Transcriptional control of the mouse alpha 2(I) collagen gene: functional deletion analysis of the promoter and evidence for cell-specific expression.
1986,
Pubmed
Schnieke,
Introduction of the human pro alpha 1(I) collagen gene into pro alpha 1(I)-deficient Mov-13 mouse cells leads to formation of functional mouse-human hybrid type I collagen.
1987,
Pubmed
Schnieke,
Embryonic lethal mutation in mice induced by retrovirus insertion into the alpha 1(I) collagen gene.
,
Pubmed
Seperack,
Somatic and germ-line reverse mutation rates of the retrovirus-induced dilute coat-color mutation of DBA mice.
1988,
Pubmed
Shinnick,
Nucleotide sequence of Moloney murine leukaemia virus.
,
Pubmed
Simchen,
Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes.
1984,
Pubmed
Soriano,
Retroviruses and insertional mutagenesis in mice: proviral integration at the Mov 34 locus leads to early embryonic death.
1987,
Pubmed
Southern,
Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
1982,
Pubmed
Spence,
Spontaneous germ line virus infection and retroviral insertional mutagenesis in eighteen transgenic Srev lines of mice.
1989,
Pubmed
Stacey,
Perinatal lethal osteogenesis imperfecta in transgenic mice bearing an engineered mutant pro-alpha 1(I) collagen gene.
1988,
Pubmed
Stewart,
De novo methylation, expression, and infectivity of retroviral genomes introduced into embryonal carcinoma cells.
1982,
Pubmed
Stoye,
Role of endogenous retroviruses as mutagens: the hairless mutation of mice.
1988,
Pubmed
Stuhlmann,
Infectivity and methylation of retroviral genomes is correlated with expression in the animal.
1981,
Pubmed
Weiher,
Transgenic mouse model of kidney disease: insertional inactivation of ubiquitously expressed gene leads to nephrotic syndrome.
1990,
Pubmed
Winston,
Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.
1984,
Pubmed
Wu,
Human-mouse interspecies collagen I heterotrimer is functional during embryonic development of Mov13 mutant mouse embryos.
1990,
Pubmed
Zachar,
A detailed developmental and structural study of the transcriptional effects of insertion of the Copia transposon into the white locus of Drosophila melanogaster.
1985,
Pubmed
van der Eb,
Assay of transforming activity of tumor virus DNA.
1980,
Pubmed
