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Mol Cell Biol
1991 Nov 01;1111:5410-6. doi: 10.1128/mcb.11.11.5410-5416.1991.
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Conserved mechanism of tRNA splicing in eukaryotes.
Zillmann M
,
Gorovsky MA
,
Phizicky EM
.
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The ligation steps of tRNA splicing in yeast and vertebrate cells have been thought to proceed by fundamentally different mechanisms. Ligation in yeast cells occurs by incorporation of an exogenous phosphate from ATP into the splice junction, with concomitant formation of a 2' phosphate at the 5' junction nucleotide. This phosphate is removed in a subsequent step which, in vitro, is catalyzed by an NAD-dependent dephosphorylating activity. In contrast, tRNA ligation in vertebrates has been reported to occur without incorporation of exogenous phosphate or formation of a 2' phosphate. We demonstrate in this study the existence of a yeast tRNA ligase-like activity in HeLa cells. Furthermore, in extracts from these cells, the entire yeastlike tRNA splicing machinery is intact, including that for cleavage, ligation, and removal of the 2' phosphate in an NAD-dependent fashion to give mature tRNA. These results argue that the mechanism of tRNA splicing is conserved among eukaryotes.
Apostol,
Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains.
1991, Pubmed
Apostol,
Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains.
1991,
Pubmed
Attardi,
Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease.
1985,
Pubmed
,
Xenbase
Baldi,
Binding and cleavage of pre-tRNA by the Xenopus splicing endonuclease: two separable steps of the intron excision reaction.
1986,
Pubmed
,
Xenbase
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Filipowicz,
RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract.
1983,
Pubmed
,
Xenbase
Filipowicz,
Origin of splice junction phosphate in tRNAs processed by HeLa cell extract.
1983,
Pubmed
,
Xenbase
Green,
The discovery of new intron-containing human tRNA genes using the polymerase chain reaction.
1990,
Pubmed
Greer,
Substrate recognition and identification of splice sites by the tRNA-splicing endonuclease and ligase from Saccharomyces cerevisiae.
1987,
Pubmed
Greer,
Mechanism of action of a yeast RNA ligase in tRNA splicing.
1983,
Pubmed
Kikuchi,
Circularization of linear viroid RNA via 2'-phosphomonoester, 3', 5'-phosphodiester bonds by a novel type of RNA ligase from wheat germ and Chlamydomonas.
1982,
Pubmed
Konarska,
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.
,
Pubmed
Konarska,
RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.
1982,
Pubmed
Laski,
Characterization of tRNA precursor splicing in mammalian extracts.
1983,
Pubmed
,
Xenbase
Lee,
Transfer RNA splicing in Saccharomyces cerevisiae. Secondary and tertiary structures of the substrates.
1985,
Pubmed
Mattoccia,
Site selection by the tRNA splicing endonuclease of Xenopus laevis.
1988,
Pubmed
,
Xenbase
McCraith,
An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule.
1991,
Pubmed
McCraith,
A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing.
1990,
Pubmed
Nishikura,
RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.
1981,
Pubmed
,
Xenbase
Peebles,
Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease.
1983,
Pubmed
Perkins,
Isolation and characterization of an RNA ligase from HeLa cells.
1985,
Pubmed
Phizicky,
Saccharomyces cerevisiae tRNA ligase. Purification of the protein and isolation of the structural gene.
1986,
Pubmed
Pick,
Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity.
1986,
Pubmed
Pick,
Purification of wheat germ RNA ligase. II. Mechanism of action of wheat germ RNA ligase.
1986,
Pubmed
Reyes,
Substrate recognition and splice site determination in yeast tRNA splicing.
1988,
Pubmed
Schwartz,
Enzymatic mechanism of an RNA ligase from wheat germ.
1983,
Pubmed
Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Stange,
Wheat germ splicing endonuclease is highly specific for plant pre-tRNAs.
1988,
Pubmed
,
Xenbase
Swerdlow,
Structure of intron-containing tRNA precursors. Analysis of solution conformation using chemical and enzymatic probes.
1984,
Pubmed
Szekely,
Intron sequence and structure requirements for tRNA splicing in Saccharomyces cerevisiae.
1988,
Pubmed
Tyc,
Ligation of endogenous tRNA 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of Chlamydomonas.
1983,
Pubmed
Winicov,
Nuclear ligation of RNA 5'-OH kinase products in tRNA.
1982,
Pubmed
van Tol,
All human tRNATyr genes contain introns as a prerequisite for pseudouridine biosynthesis in the anticodon.
1988,
Pubmed
