XB-ART-24235
Growth Factors
1992 Jan 01;74:279-88. doi: 10.3109/08977199209046410.
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Recombinant PDGF from lower vertebrates: receptor binding and immunochemical analysis with metabolically labeled growth factor.
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We used a baculovirus vector/insect host cell system to express cDNA clones of PDGF A genes from mouse and frog (Xenopus laevis). The insect host cells process PDGF A subunits from either frogs or mice into biologically active AA homodimers with yields in the range of 0.5-1.0 mg/liter of culture medium. The recombinant PDGFs can be metabolically labeled with 35S-cysteine for use in radioreceptor and radioimmunoassays. Neutralizing polyclonal antisera can be raised against the mouse and frog PDGFs. These antisera are markedly species-specific in action. However, in radioreceptor binding assays and bioassays for mitogenic activity, human, mouse and frog PDGF AA homodimers occupy and activate murine PDGF receptors with equal efficiency.
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Species referenced: Xenopus laevis
Genes referenced: pdgfa