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High level expression of a frog alpha-amidating enzyme, AE-II, in cultured cells and silkworm larvae using a Bombyx mori nuclear polyhedrosis virus expression vector.
Kobayashi J
,
Imanishi S
,
Inoue H
,
Ohsuye K
,
Yamaichi K
,
Tsuruoka N
,
Tanaka S
.
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A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. The expressed enzyme was identified predominantly in the culture medium and the hemolymph of silkworm larvae, indicating successful secretion of the expressed AE-II. The level of recombinant enzyme in the larval hemolymph at 4 days post-infection (40 micrograms/ml) was more than 100-fold the peak levels found in the culture medium (250 ng/ml). The enzyme activity in the larval hemolymph at 4 days post-infection was 3700 units/ml.
Beaudry,
Secreted alpha amidating enzymes are generated by specific posttranslational processing of precursors containing transmembrane domains.
1989, Pubmed
Beaudry,
Secreted alpha amidating enzymes are generated by specific posttranslational processing of precursors containing transmembrane domains.
1989,
Pubmed
Kato,
Two enzymes concerned in peptide hormone alpha-amidation are synthesized from a single mRNA.
1990,
Pubmed
Maeda,
Expression of foreign genes in insects using baculovirus vectors.
1989,
Pubmed
Maeda,
Production of human alpha-interferon in silkworm using a baculovirus vector.
,
Pubmed
Matsuura,
Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins.
1987,
Pubmed
Miller,
Baculoviruses as gene expression vectors.
1988,
Pubmed
Miyajima,
Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3.
1987,
Pubmed
Mizuno,
Cloning and sequence of cDNA encoding a peptide C-terminal alpha-amidating enzyme from Xenopus laevis.
1987,
Pubmed
,
Xenbase
Mizuno,
Peptide C-terminal alpha-amidating enzyme purified to homogeneity from Xenopus laevis skin.
1986,
Pubmed
,
Xenbase
Ohsuye,
Cloning of cDNA encoding a new peptide C-terminal alpha-amidating enzyme having a putative membrane-spanning domain from Xenopus laevis skin.
1988,
Pubmed
,
Xenbase
Suzuki,
Elucidation of amidating reaction mechanism by frog amidating enzyme, peptidylglycine alpha-hydroxylating monooxygenase, expressed in insect cell culture.
1990,
Pubmed
,
Xenbase
Tsujimoto,
Purification and characterization of recombinant human interleukin 5 expressed in Chinese hamster ovary cells.
1989,
Pubmed
